Neuropeptide Y Protocols
It is now clear that neuropeptide Y (NPY), the most abundant peptide present in the mammalian nervous system, is implicated in the pathophysiology of many diseases, including eating disorders, seizures, memory loss, anxiety, depression, and cardiovascular diseases, and offers major therapeutic oppor...
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| Format: | eBook |
| Language: | English |
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Totowa, NJ
Humana Press
2000, 2000
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| Edition: | 1st ed. 2000 |
| Series: | Methods in Molecular Biology
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| Online Access: | |
| Collection: | Springer Protocols Archive 1981-2004 - Collection details see MPG.ReNa |
Table of Contents:
- Cloning Techniques
- Cloning Neuropeptide Tyrosine cDNA
- Human Y1/Y5 Receptor Gene Cluster
- Human Type 2 Neuropeptide Y Receptor Gene
- Y4 Receptor in Different Species
- NeuropeptideYY5 Receptor Expression
- Homology-Based Cloning Methods
- Production of Transgenic and Knock-Out Models
- Developing Transgenic NeuropeptideY Rats
- NeuropeptideYY1 Receptor-Deficient Mice
- Antisense Technology
- Antisense Oligonucleotide Approach to Study NPY-Mediated Feeding Signal Transduction
- A G?i RNA-Antisense Expression Strategy to Investigate Coupling of Peptide YY/Neuropeptide Y Receptor to G?i
- NPY Y5 Receptor Subtype
- NPY Antisense Oligodeoxynucleotides to Study the Actions of NPY
- Techniques to Study Tissue Distribution of mRNA
- Localization of Y-Receptor Subtype mRNAs in Rat Brain by Digoxigenin Labeled In Situ Hybridization
- Central Y4 Receptor Distribution
- Combining Non-Isotopic Localization of NPY mRNA with Immunocytochemistry
- Miscellaneous Techniques
- Y Receptors Characterized by RT-PCR
- Quantification of NPY mRNA by Ribonuclease Protection Assay
- Radioligand Binding Studies