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090727 ||| eng |
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|a 9781592590827
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| 050 |
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|a QR
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| 100 |
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|a Marsh, Michael N.
|e [editor]
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| 245 |
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|a Celiac Disease
|h Elektronische Ressource
|b Methods and Protocols
|c edited by Michael N. Marsh
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| 250 |
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|a 1st ed. 2000
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| 260 |
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|a Totowa, NJ
|b Humana Press
|c 2000, 2000
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| 300 |
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|a XVI, 288 p
|b online resource
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| 505 |
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|a Celiac Disease -- Genotyping Methodologies -- From Linkage to Genes -- Linkage and the Transmission Disequilibrium Test in Complex Traits -- Extraction, Separation, and Purification of Wheat Gluten Proteins and Related Proteins of Barley, Rye, and Oats -- Structure Elucidation of Gluten-Derived Peptides by Tandem Mass Spectrometry -- Synthetic Peptide Libraries for T-Cell Epitope Identification -- Characterization of HLA-DQ-Specific Peptide-Binding Motifs -- Studies of Gliadin-Specific T-Cells in Celiac Disease -- Morphometric Analysis of Intestinal Mucosa -- Morphometry of Rectal Mucosa -- Organ Culture of Rectal Mucosa -- In Situ Hybridization -- Measurements of Cytokine mRNA Expression by Quantitative Polymerase Chain Reaction in Studies of Celiac Disease -- Immunohistochemistry in Research and Diagnosis of Celiac Disease -- Immunoassay for Detection of IgA Antitissue Transglutaminase in Patients with Celiac Disease -- Antiendomysial and Antigliadin Antibody Tests and Diagnosis of Celiac Disease -- Whole Gut Lavage Fluid Analysis
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| 653 |
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|a Microbiology
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| 041 |
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7 |
|a eng
|2 ISO 639-2
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| 989 |
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|b SPRPROT
|a Springer Protocols Archive 1981-2004
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| 490 |
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|a Methods in Molecular Medicine
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| 024 |
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|a 10.1385/1592590829
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| 856 |
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|u https://doi.org/10.1385/1592590829?nosfx=y
|x Verlag
|3 Volltext
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| 082 |
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|a 579
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| 520 |
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|a With the recent development of powerful bioanalytical techniques, research on gluten sensitivity has entered an exciting new phase. In Celiac Disease: Methods and Protocols, Professor Michael N. Marsh, a recognized world authority on this condition, together with a team of other expert laboratory/clinical investigators from around the world present a collection of these state-of-the-art techniques for studying the biology and immunopathology of celiac disease. Both novice and experienced researcher will find in this collection detailed step-by-step methods for cloning lymphocytes, creating gene/peptide libraries, and performing genotyping, linkage, and positional cloning. Also included are techniques for determining the peptide structure of HLA-bound material (tandem mass spectroscopy), computerized morphometry, in situ hybridization, organ culture, and monoclonal AB assays. Comprehensive and cutting-edge, Celiac Disease: Methods and Protocols provides all experimental and clinical investigators with the essential core of readily reproducible methods necessary for successful work on celiac disease today
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