Calpain Methods and Protocols
In Calpain Methods and Protocols, John S. Elce and a seasoned team of principal investigators present a set of proven and easily followed protocols for studying calpain. The methods include in vitro techniques for the detection, expression, purification, and assay of µ- and m-calpain, supplemented w...
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| Format: | eBook |
| Language: | English |
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Totowa, NJ
Humana Press
2000, 2000
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| Edition: | 1st ed. 2000 |
| Series: | Methods in Molecular Biology
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| Subjects: | |
| Online Access: | |
| Collection: | Springer Protocols Archive 1981-2004 - Collection details see MPG.ReNa |
Table of Contents:
- The Role of Calpain in Neurofilament Protein Degradation Associated With Spinal Cord Injury
- Calpain-Mediated Truncation of Glutamate Ionotropic Receptors
- Concurrent Assessment of Calpain and Caspase-3 Activity by Means of Western Blots of Protease-Specific Spectrin Breakdown Products
- Rat Renal Proximal Tubules, Hypoxia, lonomycin, and Calpain
- Calpain Activity in Rat Renal Proximal Tubules
- Calpain Activity in Rat Renal Proximal Tubules
- Cellular In Vivo Assay of Calpain Activity Using a Fluorescent Substrate
- Calpain Methods in Hepatic Ischemia-Reperfusion Injury
- Myocardial Ischemia-Reperfusion Injury and Proteolysis of Fodrin, Ankyrin, and Calpastatin
- Calpains in the Lens and Cataractogenesis
- Specific Substrates
- Proteolysis of Cortactin by Calpain in Platelets and In Vitro
- Proteolysis of p53 Protein by Ubiquitous Calpains
- Modulation of Calpain-Mediated Protein Kinase C Activation Within Intact Cells
- Preparation of Calpastatin Samples for Western Blotting
- Isolation and Characterization of Calpain Activator Protein from Bovine Brain
- Assays of Calpain
- Calpain Zymography with Casein or Fluorescein Isothiocyanate Casein
- Casein Zymogram Assessment of ?-Calpain and m-Calpain Activity After Traumatic Brain Injury in the Rat In Vivo
- Fluorescence Measurements of Ca2+ Binding to Domain VI of Calpain
- Kinetic Analysis of Human ?-Calpain Autolysis
- A Sensitive and Continuous Fluorometric Activity Assay Using a Natural Substrate
- Measurement of Calpain Activity In Vitro and In Situ Using a Fluorescent Compound andTau as Substrates
- Localization of Calpain by Immunofluorescence in Adherent Cells
- A Radioimmunologic Technique for Assessing Calpain Activation in Cells
- Specific Tissues
- Calpains and Myogenesis
- Calpastatin (the Endogenous Calpain Inhibitor) and Membrane Protein Degradation in Cell Fusion
- Strategies for Regulating Calpain Activities in Living Cells
- Assays of Apoptosis
- Purification of Components of the Calpain System
- Purification of µ- and m-Calpain and Calpastatin from Animal Tissues
- A Simple Protocol for Separation and Assay of µ-Calpain, m-Calpain and Calpastatin from Small Tissue Samples
- Purification and Quantification of Calcium-Activated Neutral Proteases I and II and Novel Isoforms from Cultured Osteoblastic Cells by Ion-Exchange Fast Protein Liquid Chromatography
- Purification of Calpain by Affinity Chromatography on Reactive Red-Agarose or on Casein-Sepharose
- Affinity Purification of ?-Calpain from Erythrocytes on an Immobilized Peptide from the Plasma Membrane Calcium Pump
- Expression of m-Calpain in Escherichia coli
- Purification and Characterization of Crustacean Calpain-like Proteinases
- Drosophila Cal pains
- Molecular Analysis of p94 and Its Application to Diagnosis of Limb Girdle Muscular Dystrophy Type 2A
- Purification of Recombinant Calpastatin Expressed in Escherichia coli