DNA Methylation Protocols

Since methylation is now understood to be integral to DNA replication and transcription, with abnormalities frequently found to occur during tumorigenesis, the number of breakthrough techniques for methylation's study has sharply increased. In DNA Methylation Protocols, an internationally well-...

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Bibliographic Details
Other Authors: Mills, Ken I. (Editor), Ramsahoye, Bernie H. (Editor)
Format: eBook
Language:English
Published: Totowa, NJ Humana Press 2002, 2002
Edition:1st ed. 2002
Series:Methods in Molecular Biology
Subjects:
Online Access:
Collection: Springer Protocols Archive 1981-2004 - Collection details see MPG.ReNa
Table of Contents:
  • Overview
  • Nearest-Neighbor Analysis
  • Measurement of Genome-Wide DNA Cytosine-5 Methylation by Reversed-Phase High-Pressure Liquid Chromatography
  • Methylation Analysis by Chemical DNA Sequencing
  • Methylation-Sensitive Restriction Fingerprinting
  • Restriction Landmark Genome Scanning
  • Combined Bisulfite Restriction Analysis (COBRA)
  • Differential Methylation Hybridization Using CpG Island Arrays
  • Methylated CpG Island Amplification for Methylation Analysis and Cloning Differentially Methylated Sequences
  • Isolation of CpG Islands Using a Methyl-CpG Binding Column
  • Purification of MeCP2-Containing Deacetylase from Xenopus laevis
  • DNA-Methylation Analysis by the Bisulfite-Assisted Genomic Sequencing Method
  • Measuring DNA Demethylase Activity In Vitro
  • Extracting DNA Demethylase Activity from Mammalian Cells