Calcium Signaling Protocols
In Calcium Signaling Protocols, David Lambert and a panel of leading authorities present a wide range of experimental protocols for studying Ca2+ signaling. These optimized techniques cover the more common applications, including 45Ca2+ flux measurements, and basic fluorometric technology, as well a...
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| Format: | eBook |
| Language: | English |
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Totowa, NJ
Humana Press
1999, 1999
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| Edition: | 1st ed. 1999 |
| Series: | Methods in Molecular Biology
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| Online Access: | |
| Collection: | Springer Protocols Archive 1981-2004 - Collection details see MPG.ReNa |
Table of Contents:
- General
- Fluorescent Measurement of [Ca2+]c
- Measurement of [Ca2+]i in Whole Cell Suspensions Using Fura-2
- Measurement of [Ca2+]i in Cell Suspensions Using Indo-1
- Specialist Measurement Systems
- Confocal Microscopy
- Measurement of Intracellular Calcium Concentration Using Confocal Microscopy
- Single Cell and Subcellular Measurement of Intracellular Ca2+ Concentration ([Ca2+]i)
- Measurement of [Ca2+] Using the Fluorometric Imaging Plate Reader (FLIPR)
- Nonelectrophysiological measurement of Ca2+ channel activity
- Measurement of Ca2+ Entry Using 45Ca2+
- Measurement of [3H]PN200-110 and [125I]?-Conotoxin MVIIA Binding
- Measurement of Ins(1,4,5)P3 and Ca2+ Release from Intracellular Stores
- Measurement of Inositol (Poly)phosphate Formation Using [3H]Inositol Labeling Protocols in Permeabilized Cells
- Measurement of Inositol(1,4,5)trisphosphate Using a Stereospecific Radioreceptor Mass Assay
- Measurement of Calcium Fluxes in Permeabilized Cells Using a 45Ca2+ Uptake and Release Assay
- Microinjection of myo-Inositol(1,4,5)trisphosphate and Other Calcium-Mobilizing Agents into Intact Adherent Cells
- Photolysis of Caged Calcium Using a Low-Cost Flash Unit
- Measurement of Ca2+ Flux Through Ins(1,4,5)P3 Receptor-Ca2+ Channels in Lipid Bilayers (“Dip-Tip” and “Schindler” Methodology)
- Continuous Fluorescent Monitoring of Cellular Calcium Fluxe
- Measurement of Free [Ca2+] Changes in Agonist-Sensitive Internal Stores Using Compartmentalized Fluorescent Indicators
- Specialist Measurement Techniques
- Measurement of [Ca2+]i in Smooth Muscle Strips Using Front-Surface Fluorimetry
- Measurement of Calcium and Movement in Heart Cells
- Simultaneous Analysis of Intracellular pH and Ca2+ from Cell Populations
- Measurement of Cytosolic-Free Ca2+ in Plant Tissue
- Ca2+ Sensitive Targets
- Assay and Purification of Calmodulin-Dependent Protein Kinase
- Measurement of Ca2+-ATPase Activity (in PMCA and SERCA1)