Animal Cell Biotechnology Methods and Protocols
In Animal Cell Biotechnology, scientists with long-refined expertise describe cutting-edge techniques for the production of therapeutic proteins and vaccines. Capturing the major advances that have occurred in both the science and the technology of these biopharmaceuticals, this important book cover...
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| Format: | eBook |
| Language: | English |
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Totowa, NJ
Humana Press
1999, 1999
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| Edition: | 1st ed. 1999 |
| Series: | Methods in Biotechnology
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| Online Access: | |
| Collection: | Springer Protocols Archive 1981-2004 - Collection details see MPG.ReNa |
Table of Contents:
- Enabling Technologies
- Setting Up a New Cell Culture Laboratory
- Adaptation of Mammalian Cells to Growth in Serum-Free Media
- Viral Evaluation of Animal Cell Lines Used in Biotechnology
- Molecular Methods
- Optimizing Gene Expression in Mammalian Cells
- Cytogenetic Characterization of Recombinant Cells
- Immortalization Strategies for Mammalian Cells
- Mouse Monoclonal Antibodies
- Cell Bank Preparation and Characterization
- DNA Fingerprinting and Characterization of Animal Cells
- Cell Evaluation Protocols
- Cell Counting and Viability Measurements
- Monitoring Animal Cell Growth and Productivity by Flow Cytometry
- Measurement of Cell Death in Culture
- Nuclear Magnetic Resonance Methods of Monitoring Cell Metabolism
- Specialist Techniques
- Culturing Animal Cells in Fluidized Bed Reactors
- GPI-Anchored Fusion Proteins
- Harvesting GPI-Anchored Proteins From CHO Cells
- Hematopoietic Cells for Cellular and Gene Therapy
- Hematopoietic Cells for Cellular and Gene Therapy
- Cytotoxicity Testing Using Cell Lines
- Product Evaluation Protocols
- Measuring the Folding Dynamics of Recombinant Proteins Secreted from Mammalian Cells
- Control of Proteolysis in Cell Culture
- Monitoring Recombinant Glycoprotein Heterogeneity
- Measurement of Cell-Culture Glycosidase Activity