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090727 ||| eng |
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|a 9781592595808
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| 050 |
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4 |
|a QD415-436
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| 100 |
1 |
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|a Phillips, Ian R.
|e [editor]
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| 245 |
0 |
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|a Cytochrome P450 Protocols
|h Elektronische Ressource
|c edited by Ian R. Phillips, Elizabeth A. Shephard
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| 250 |
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|a 1st ed. 1998
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| 260 |
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|a Totowa, NJ
|b Humana Press
|c 1998, 1998
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| 300 |
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|a XIV, 482 p. 49 illus
|b online resource
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0 |
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|a Determination of CYP2C9-Catalyzed Diclofenac 4?-Hydroxylation by High-Performance Liquid Chromatography -- CYP2Cl g-Mediated (S)-Mephenytoin 4’-Hydroxylation Assayed by High-Performance Liquid Chromatography with Radiometric Detection -- CYP2D6-Dependent Bufuralol 1’-Hydroxylation Assayed by Reversed-Phase lon-Pair High-Performance Liquid Chromatography with Fluorescence Detection -- Spectrophotometric Analysis of Human CYP2E1-Catalyzed p-Nitrophenol Hydroxylation -- Thin-Layer Chromatographic Analysis of Human CYP3A-Catalyzed Testosterone 6?-Hydroxylation -- Determination of CYP4A11-Catalyzed Lauric Acid 12-Hydroxylation by High-Performance Liquid Chromatography with Radiometric Detection -- An Isocratic High-Performance Liquid Chromatographic Assay for CYP7Al-Catalyzed Cholesterol 7?-Hyciroxylation -- Use of 7-Ethoxycoumarinto Monitor Multiple Enzymesin the Human CYP1, CYP2, and CYP3 Families -- Expression of Eukaryotic Cytochromes P450 in E. coli --
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| 505 |
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|a Expression of Mammalian Cytochromes P450 in Yeast -- Expression of Cytochromes P450 in a Baculovirus System -- Expression of Cytochromes P450 in Mammalian Cells -- Monoclonal Antibodies to Cytochromes P450 -- Targeting Antipeptide Antibodies Towards Cytochrome P450 Enzymes -- Signals for Retention of Cytochrome P450 in the Endoplasmic Reticulum -- Quantification of Cytochrome P450 Gene Expression by RNase Protection Analysis -- Hepatocyte Cultures in Drug Metabolism and Toxicological Research and Testing -- Isolation of Rat Hepatocytes -- Collagen-Gel Cultures of Rat Hepatocytes: -- Rat Hepatocyte Cultures -- Isolation of Periportal and Pericentral Hepatocytes -- Perifusion Culture of Hepatocytes -- Human Hepatocyte Culture -- Reconstruction of Human Epidermis In Vitro -- Gene Transfer and Expression -- Lipid-Mediated Gene Transfer into Normal Adult Human Hepatocytes in Primary Culture --
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|a The Role of Methods in the Discovery of the Cytochromes P450 -- Cytochrome P450 Nomenclature -- Spectral Analyses of Cytochromes P450 -- Purification of Cytochromes P450 -- Isolation and Purification of Constitutive Forms of Microsomal Cytochrome P450 -- Purification of Extrahepatic Cytochromes P450 -- Purification of Cytochromes P450: Products of Bacterial Recombinant Expression Systems -- Cytochrome P450 Reconstitution Systems -- Catalytic Assays for Human Cytochrome P450 -- Enzymatic Analysis of cDNA-Expressed Human CYP1A1, CYP1A2, and CYP1B1 with 7-Ethoxyresorufin as Substrate -- Spectrofluorometric Analysis of CYP2A6-Catalyzed Coumarin 7-Hydroxylation -- Determination of the CYP2B6 Component of 7-Ethoxy-4-Trifluoromethylcoumarin O-Deethylation Activity in Human Liver Microsomes -- High-Performance Liquid Chromatographic Analysis of CYP2CSCatalyzed Paclitaxel 6?-Hydroxylation --
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| 505 |
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|a Identification of Regulatory Protein- Binding Sites in Cytochrome P450 Genes by Means of the Gel- Retardation Assay -- Cytochrome P450 Gene Regulation -- Analysis of Cytochrome P450 Polymorphisms -- Induction of Cytochrome P4501A (CYP1A) in Fish A Biomarker for Environmental Pollution -- Generation of Allelic Series in Mice by Gene Targeting
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| 653 |
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|a Biochemistry
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| 700 |
1 |
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|a Shephard, Elizabeth A.
|e [editor]
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| 041 |
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7 |
|a eng
|2 ISO 639-2
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| 989 |
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|b SPRPROT
|a Springer Protocols Archive 1981-2004
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| 490 |
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|a Methods in Molecular Biology
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| 028 |
5 |
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|a 10.1385/0896035190
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| 856 |
4 |
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|u https://doi.org/10.1385/0896035190?nosfx=y
|x Verlag
|3 Volltext
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| 082 |
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|a 572
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| 520 |
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|a In Cytochrome P450 Protocols, Ian Phillips and Elizabeth Shephard assemble a comprehensive collection of cutting-edge techniques for the investigation of cytochromes P450. Described in detail by hands-on experimentalists for easy reproducibility, these methods include spectral analysis, purification and enzymatic assays, expression in heterologous systems, and the production and use of antibodies, as well as methods for quantification of gene expression, transfection of hepatocytes, and for the investigation of DNA-protein interactions and genetic polymorphisms. In addition, because of the growing importance of in vitro systems in pharmacological toxicology, the book contains techniques for the culture of rodent and human hepatocytes and human epidermis. Cytochrome P450 induction as a biomarker for environmental pollution and the generation of mice with targeted gene disruptions complete this exhaustive collection of core techniques. Cytochrome P450 Protocols includes in one volume both state-of-the-art and classic methods that have not been superseded but remain extremely useful. The collection provides both novice and experienced researchers across many fields-toxicology, pharmacology, environmental biology, biochemistry, and molecular biology-all the tools needed to elucidate the crucial biological role played by cytochromes P450 in the metabolism of therapeutic drugs, chemical carcinogens, and environmental pollutants
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