DNA Topoisomerase Protocols Volume II: Enzymology and Drugs
DNA Topoisomerase Protocols, II: Enzymology and Drugs brings together an unprecedented collection of cutting-edge experimental protocols for investigating the catalytic activities of DNA topoisomerases, as well as their specific interactions with topoisomerase-targeted antitumor and antibacterial dr...
| Other Authors: | , |
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| Format: | eBook |
| Language: | English |
| Published: |
Totowa, NJ
Humana Press
2001, 2001
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| Edition: | 1st ed. 2001 |
| Series: | Methods in Molecular Biology
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| Subjects: | |
| Online Access: | |
| Collection: | Springer Protocols Archive 1981-2004 - Collection details see MPG.ReNa |
Table of Contents:
- Assaying DNA Topoisomerase I Relaxation Activity
- DNA Topoisomerase II-Catalyzed DNA Decatenation
- Plasmid DNA Supercoiling by DNA Gyrase
- Analyzing Reverse Gyrase Activity
- Topoisomerase II-Catalyzed ATP Hydrolysis as Monitored by Thin-Layer Chromatography
- Use of a Real-Time, Coupled Assay to Measure the ATPase Activity of DNA Topoisomerase II
- Analysis of Topoisomerase-DNA Interactions by Electrophoretic Mobility Shift Assay
- Filter Binding Assays for Topoisomerase—DNA Complexes
- DNATopoisomerase I-Mediated Nicking of Circular Duplex DNA
- Analysis of Cleavage Complexes Using Reactive Inhibitor Derivatives
- Uncoupling of Topoisomerase-Mediated DNA Cleavage and Religation
- Synthesis and Use of DNA Containing a 5?-Bridging Phosphorothioate as a Suicide Substrate for Type I DNA Topoisomerases
- Isolation of Covalent Enzyme-DNA Complexes
- ICE Bioassay
- DNA-Unwinding Test Using Eukaryotic DNA Topoisomerase I
- Drug—DNA Interactions
- Quinolone Interactions with DNA and DNA Gyrase
- Bactericidal Assays for Fluoroquinolones
- Drug Toxicity in E. coli Cells Expressing Human Topoisomerase I
- Drug-Induced Cytotoxicity in Tissue Culture
- Analysis of Drug Uptake-Flow Cytometry
- Cell-Cycle Analysis of Drug-Treated Cells
- Assaying Drug-Induced Apoptosis
- Single-Strand Conformational Polymorphism Analysis of DNA Topoisomerases I and II
- PCR-Based Cloning of DNA Topoisomerase Genes
- Topoisomerase II-Catalyzed Relaxation and Catenation of Plasmid DNA
- Topoisomerase II-Mediated Cleavage of Plasmid DNA
- Drug-induced Stabilization of Covalent DNA Topoisomerase I-DNA Intermediates
- Studying DNATopoisomerase I-Targeted Drugs In the Yeast
- Yeast Systems for Demonstrating the Targets of Anti-Topoisomerase II Agents