Immunochemical Protocols
This much anticipated second edition of the best selling Immunological Protocols provides a user-friendly, up-to-date handbook of reliable immunochemical techniques optimized for ready use by molecular biologists. It covers the breadth of relevant established methods-from protein blotting and immuno...
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| Format: | eBook |
| Language: | English |
| Published: |
Totowa, NJ
Humana Press
1998, 1998
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| Edition: | 2nd ed. 1998 |
| Series: | Methods in Molecular Biology
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| Subjects: | |
| Online Access: | |
| Collection: | Springer Protocols Archive 1981-2004 - Collection details see MPG.ReNa |
Table of Contents:
- Preparation of Avidin Conjugates
- Enhanced Chemiluminescence Immunoassay
- Nonradioactive Methods for Visualization of Protein Blots
- Colloidal Gold Staining and Immunoprobing on the Same Western Blot
- Erasure of Western Blots After Autoradiographic or Chemiluminescent Detection
- Colloidal Gold Staining and Immunodetection in 2D Protein Mapping
- Enzyme-Antienzyme Method for Immunohistochemistry
- Immunohistochemical Detection of Bromodeoxyuridine-Labeled Nuclei for In Vivo Cell Kinetic Studies
- Cell Kinetic Studies Using a Monoclonal Antibody to Bromodeoxyuridine
- Immunohistochemical Detection of Cells in the Division Cycle Using Antibodies to Proliferating Cell Nuclear Antigen (PCNA)
- Double Label Immunohistochemistry on Tissue Sections Using Alkaline Phosphatase and Peroxidase Conjugates
- Preparation of Colloidal Gold Probes
- Immunogold Probes in Light Microscopy
- Immunogold Probes in Electron Microscopy
- Electron Microscopic-Silver Enhancement for Double Labeling with Antibodies Raised in the Same Species
- Flow Cytometric Analysis of Cell Surface Antigen Density
- Multiple Immunofluorescence Analysis of Cells Using Flow Cytometry
- Flow Cytometric Methods of Analyzing Apoptotic Cells
- Flow Cytometric Detection of Proliferation-Associated Antigens, PCNA and Ki-67
- Sorting of Human Peripheral Blood T-Cell Subsets Using Immunomagnetic Beads
- Preparation and Use of Nonradioactive Hybridization Probes
- Cellular Human and Viral DNA Detection by Nonisotopic In Situ Hybridization
- Biotinylated Probes in Colony Hybridization
- Nonisotopic In Situ Hybridization
- Expression of Recombinant Antibody Fusion Proteins in E. coli
- Expression of Antibody Fusion Proteins in Mammalian Cells
- Antibody Screening of Bacteriophage ? gt-11 DNA Expression Libraries
- Display of Antibody Chains on Filamentous Bacteriophage
- Production of Polyclonal Antisera
- Raising Polyclonal Antibodies Using Nitrocellulose-Bound Antigen
- Production and Characterization of Antibodies Against Synthetic Peptides
- Preparation and Testing of Monoclonal Antibodies to Recombinant Proteins
- A Simple Hollow-Fiber Bioreactor for the “In-House” Production of Monoclonal Antibodies
- Screening of Monoclonal Antibodies Using Antigens Labeled with Acetylcholinesterase
- Synthesis of Peptides for Use as Immunogens
- Use of Proteins Blotted to Polyvinylidene Difluoride Membranes as Immunogens
- Purification of Glycoproteins and Their Use as Immunogens
- IgG Purification
- Purification of Monoclonal Antibodies
- Production of Bispecif ic and Trispecific F(ab)2 and F(ab)3 Antibody Derivatives
- Preparation of Cytotoxic Antibody—Toxin Conjugates
- Immunoaffinity Purification and Quantification of Antibody—Toxin Conjugates
- Competitive ELISA
- Epitope Mapping
- Coupling of Antibodies with Biotin
- Production of Human Fab Antibody Fragments from Phage Display Libraries
- Antibodies from Phage Display Libraries as Immunochemical Reagents