Drug'DNA Interaction Protocols
In Drug-DNA Interactions, expert researchers describe powerful molecular techniques designed to illuminate and explore the interaction of drugs and ligands with DNA, often perfected in their own laboratories, . These easily reproducible methods involve sequence recognition properties, as well as phy...
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| Format: | eBook |
| Language: | English |
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Totowa, NJ
Humana Press
1997, 1997
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| Edition: | 1st ed. 1997 |
| Series: | Methods in Molecular Biology
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| Online Access: | |
| Collection: | Springer Protocols Archive 1981-2004 - Collection details see MPG.ReNa |
Table of Contents:
- DNase I Footprinting
- Quantitative DNA Footprinting
- Uranyl Photoprobing of DNA Structures and Drug–DNA Complexes
- Diethylpyrocarbonate and Osmium Tetroxide as Probes for Drug-Induced Changes in DNA Conformation In Vitro
- Footprinting Studies with Nucleosome-Bound DNA
- A Gel Mobility Shift Assay for Probing the Effect of Drug–DNA Adducts on DNA-Binding Proteins
- An Oligonucleotide Crosslinking Assay for the Analysis of Individual Drug–Binding Sites
- DNA Polymerase Inhibition Assay (PIA) for the Detection of Drug–DNA Interactions
- Transcriptional Footprinting of Drug–DNA Interactions
- Determination of the DNA Sequence Specificity of Alkylation Damage Using Cleavage-Based Assays
- PCR-Based Methods for Detecting DNA Damage and Its Repair at the Subgene and Single Nucleotide Levels in Cells
- Analysis of DNA-Binding Antibodies
- Immunofluorescent Staining of Chromosomes with DNA-Binding Antibodies
- Optical Absorbance and Fluorescence Techniques for Measuring DNA–Drug Interactions
- Evaluation of Drug-Nucleic Acid Interactions by Thermal Melting Curves
- Electric Dichroism
- Calorimetric Techniques for Studying Drug–DNA Interactions
- Methods for the Studies of Drug Dissociation from DNA.