Arabidopsis Protocols
n Arabidopsis Protocols internationally recognized experts offer a comprehensive collection of significant new methods for gene mapping, cloning, and reverse genetics, as well as updated standard protocols for work in Arabidopsis genetics and molecular biology. These time-tested methods range from t...
| Other Authors: | , |
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| Format: | eBook |
| Language: | English |
| Published: |
Totowa, NJ
Humana Press
1998, 1998
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| Edition: | 1st ed. 1998 |
| Series: | Methods in Molecular Biology
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| Subjects: | |
| Online Access: | |
| Collection: | Springer Protocols Archive 1981-2004 - Collection details see MPG.ReNa |
Table of Contents:
- AFLP? Fingerprinting of Arabidopsis
- Building a High-Density Genetic Map Using the AFLP? Technology
- 21. Use of Cleaved Amplified Polymorphic Sequences (CAPS) as Genetic Markers in Arabidopsis thahna
- Mapping Mutations with ARMS
- 23. Mapping Cloned Sequences on YACs
- Transient and Stable Transformation
- Transient Gene Expression in Protoplasts of Arabidopsis thaliana
- Transient Expression of Foreign Genes in Tissues of Arabidopsis thaliana by Bombardment-Mediated Transformation
- Root Transformation by Agrobacterium tumefaciens
- Transformation of Arabidopsis thaliana C24 Leaf Discs by Agrobacterium tumefaciens
- In Planta AgrobacteriumMediated Transformation of Adult Arabidopsis thaliana Plants by Vacuum Infiltration
- PEG-Mediated Protoplast Transformation with Naked DNA
- VI Gene Cloning Strategies
- 30 Cloning Genes of Arabidopsis thaliana by Chromosome Walking
- Chromosome Landing Using an AFLPTWased Strategy
- Transposon Tagging with AC/Ds in Arabidopsis
- Arabidopsis Culture
- Growth of Plants and Preservation of Seeds
- Sterile Techniques in Arabidopsis
- Control of Pests and Diseases of Arabidopsis
- Establishment and Maintenance of Cell Suspension Cultures
- Callus Culture and Regeneration
- Protoplast Isolation, Culture, and Regeneration
- Purification of Subcellular Organelles and Macromolecules
- Preparation of Physiologically Active Chloroplasts from Arabidopsis
- Purification of Mitochondria from Arabidopsis
- Preparation of DNA from Arabidopsis
- High Molecular Weight DNA Extraction from Arabidopsis
- Chloroplast DNA Isolation
- 12 Purification of Mitochondrial DNA from Green Tissues of Arabidopsis
- Preparation of RNA
- Mutagenesis and Genetic Analysis
- 14Seed Mutagenesis of Arabidopsis
- Genetic Analysis
- Cytogenetic Analysis of Arabidopsis
- PCR-Based Identification of T-DNA Insertion Mutants
- Gene Mapping in Arabidopsis
- The Use of Recombinant Inbred Lines (RILs) for Genetic Mapping
- 33. Transposon Tagging with the En-l System
- Cloning Genes from T-DNA Tagged Mutants
- Gene Expression Analyses
- In Situ Hybridization
- Whole-Mount In Situ Hybridization in Plants
- Bacterial and Coelenterate Luciferases as Reporter Genes in Plant Cells
- P-Glucuronidase Enzyme Histochemistry on Semithin Sections of Plastic-Embedded Arabidopsis Explants
- In Situ Hybridization to RNA in Whole Arabidopsis Plants
- In Vivo Footprinting in Arabidopsis