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Plant Virology Protocols From Virus Isolation to Transgenic Resistance

Plant Virology Protocols offers for the first time a comprehensive collection of state-of-the-art techniques for generating transgenic plants that are resistant to plant viruses via the cloning and expression of the coat protein gene. Its unfailingly reproducible methods, perfected by hands-on maste...

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Bibliographic Details
Other Authors: Foster, Gary D. (Editor), Taylor, Sally (Editor)
Format: eBook
Language:English
Published: Totowa, NJ Humana Press 1998, 1998
Edition:1st ed. 1998
Series:Methods in Molecular Biology
Subjects:
Botany
Plant Science
Online Access:
Collection: Springer Protocols Archive 1981-2004 - Collection details see MPG.ReNa
Table of Contents:
  • PCR Analysis of Transgenic Tobacco Plants
  • Southern Analysis of Transgenic Tobacco Plants
  • Detection and Quantification of Transcript RNA in Transgenic Plants Using Digoxigenin-Labeled cDNA Probes
  • Western Analysis of Transgenic Plants
  • Evaluation of Resistance
  • Assaying Levels of Plant Virus by ELISA
  • Detection of Plant RNA Viruses by Nonisotopic Dot-Blot Hybridization
  • Detection and Quantification of Plant Viruses by PCR
  • Assaying Levels of Virus with Local Lesion Hosts
  • Field Testing Resistance of Transgenic Plants
  • Agronomic Performance of Transgenic Plants
  • Mechanisms of Resistance
  • Mechanisms of Resistance
  • Mechanisms of RNA-Mediated Resistance to Plant Viruses
  • Detection of Risks Associated with Coat Protein Transgenics
  • Potential Benefits of the Transgenic Control of Plant Viruses in the United Kingdom
  • Carmovirus Isolation and RNA Extraction
  • Tymovirus Isolation and Genomic RNA Extraction
  • Tombusvirus Isolation and RNA Extraction
  • Luteovirus Isolation and RNA Extraction
  • Cloning of Coat Protein Genes
  • RNA Analysis
  • RNA Fractionation by Density Gradient Centrifugation
  • cDNA Library Construction for the Lambda ZAP®-Based Vectors
  • PCR Cloning of Coat Protein Genes
  • Antibody Production
  • Expression Library Screening
  • In Vitro Transcription and Translation
  • Analysis of Coat Protein Expression Cassettes in Protoplasts
  • DNA Sequencing
  • Computer Analysis of Amino Acid Sequences
  • Plant Transformation and Primary Analysis
  • Preparation of Coat Protein-Containing Binary Vectors for Use in Agrobacterium-Mediated Transformation
  • Potato Transformation
  • Transformation of Tomato
  • Tobacco Transformation
  • Genetic Transformation of Wheat
  • Production of Transgenic Rice (Oryza sativa subspecies japonica cv. Taipei 309)
  • Molecular Analysis of Transgenic Rice
  • to Plant Virology
  • to Classical Crossprotection
  • History of Coat Protein-Mediated Protection
  • Virus Isolation, RNA Extraction, and Location of Coat Protein Genes
  • Geminivirus Isolation and DNA Extraction
  • Caulimovirus Isolation and DNA Extraction
  • Reovirus Isolation and RNA Extraction
  • Procedures for Plant Rhabdovirus Purification, Polyribosome Isolation, and Replicase Extraction
  • Hordeivirus Isolation and RNA Extraction
  • Furovirus Isolation and RNA Extraction
  • Tobravirus Isolation and RNA Extraction
  • Tobamovirus Isolation and RNA Extraction
  • Potexvirus Isolation and RNA Extraction
  • Carlavirus Isolation and RNA Extraction
  • Potyvirus Isolation and RNA Extraction
  • Trichovirus Isolation and RNA Extraction
  • Ilarvirus Isolation and RNA Extraction
  • Bromovirus Isolation and RNA Extraction
  • Cucumovirus Isolation and RNA Extraction
  • Nepovirus Isolation and RNA Extraction
  • Comovirus Isolation and RNA Extraction

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