Gene Targeting Protocols
In Gene Targeting Protocols, a panel of innovative investigators present in readily reproducible detail the latest techniques for gene replacement, gene knockout, and gene repair in both animals and plants. These expert researchers review the best gene transfer vehicles (liposomes, PEI, and EPD), di...
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| Format: | eBook |
| Language: | English |
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Totowa, NJ
Humana Press
2000, 2000
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| Edition: | 1st ed. 2000 |
| Series: | Methods in Molecular Biology
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| Online Access: | |
| Collection: | Springer Protocols Archive 1981-2004 - Collection details see MPG.ReNa |
Table of Contents:
- Nucleic Acid Transfer Using Cationic Lipids
- Optimizing Polyethylenimine-Based Gene Transfer into Mammalian Brain for Analysis of Promoter Regulation and Protein Function
- Gene Transfer and Drug Delivery by Electronic Pulse Delivery
- Strategies for Improving the Frequency and Assessment of Homologous Recombination
- Effective Gene Transfer Using Viral Vectors Based on SV40
- Rapid Generation of Isogenic Mammalian Cell Lines Expressing Recombinant Transgenes by Use of Cre Recombinase
- Site-Directed Alteration of Genomic DNA by Small-Fragment Homologous Replacement
- Mutation Correction by Homologous Recombination with an Adenovirus Vector
- Site-Specific Targeting of DNA Plasmids to Chromosome 19 Using AAV Cis and Trans Sequences
- Adeno-Associated Virus Based Gene Therapy in Skeletal Muscle
- Rapid Establishment of Myeloma Cell Lines Expressing Fab(Tac)-Protamine, a Targetable Protein Vector, Directed Against High-Affinity ?-Chain of Human Interleukin-2 Receptor
- EBV-Derived Episomes to Probe Chromatin Structure and Gene Expression in Human Cells
- Triplex-Directed Site-Specific Genome Modification
- Use of Quantitative Ligation-Mediated Polymerase Chain Reaction to Detect Gene Targeting by Alkylating Oligodeoxynucleotides
- Gene Targeting in Plants via Site-Directed Mutagenesis
- Antisense Oligonucleotides as Modulators of Pre-mRNA Splicing