PCR Sequencing Protocols
Advances in bioscience research usually arise as a result of the continu ing refinement of existing technologies. However, there are a number of occa sions v rhere newly developed methodologies have a profound effect on nearly all areas of research. Frequently these are techniques that are elegant...
| Other Authors: | |
|---|---|
| Format: | eBook |
| Language: | English |
| Published: |
Totowa, NJ
Humana Press
1996, 1996
|
| Edition: | 1st ed. 1996 |
| Series: | Methods in Molecular Biology
|
| Subjects: | |
| Online Access: | |
| Collection: | Springer Protocols Archive 1981-2004 - Collection details see MPG.ReNa |
Table of Contents:
- Preparation and Analysis of DNA Sequencing Gels
- Purification of PCR Products from Agarose Gels for Direct Sequencing
- Enzymatic Fluorescence and Biotin Labeling of Primers for PCR Sequencing
- Direct Sequencing of Double-Stranded PCR Products with the Sequenase Kit and [?-35 S] dATP
- Direct Sequencing by Thermal Asymmetric PCR
- Rapid Sequencing of cDNA Clones Direct Sequencing Using Sequential Linear/Asymmetric PCR
- Direct Sequencing of PCR Products Using Chemiluminescent Detection
- Direct DNA Sequencing of PCR Products Using Magnetic Beads
- Affinity Capture and Solid-Phase Sequencing of Biotinylated PCR Products
- Analysis of Nucleotide Sequence Variations by Solid-Phase Minisequencing
- Nonradioactive PCR Sequencing Using Digoxigenin
- Silver Sequencing™
- Direct Sequencing of PCR Products with DNA-Binding Proteins
- PCR Sequencing with the Aid of Detergents
- Direct Sequencing with Highly Degenerate and lnosine-Containing Primers
- Determination of Unknown Genomic Sequences Without Cloning
- DNA Sequencing by the Chemical Method
- Direct PCR Sequencing with Denaturants (Formamide)
- Efficient PCR Production of Single-Stranded DNA Sequencing Templates
- Preparation and Direct Automated Cycle Sequencing of PCR Products
- Solid-Phase Automated Sequencing of PCR-Amplified Genomic DNA
- Cloning PCR Products for Sequencing in M13 Vectors
- Sequencing PCR Products Cloned into M13 Vectors
- Genomic Amplification with Transcript Sequencing (GAWTS)
- DNA Rescue by the Vectorette Method
- Sequencing of (dA:dT) Cloned Mixed PCR Products from Microbial Populations