Patch-Clamp Applications and Protocols
E. Neher and B. Sakman were the first to monitor the opening and closing of single ion channels and membranes by conductance measurements. In 1976, they used firepolished micropipets with a tip diameter of 3-5 pm to record currents from a small patch of the membranbe of sk- etal muscles, thereby dec...
| Other Authors: | , , |
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| Format: | eBook |
| Language: | English |
| Published: |
Totowa, NJ
Humana Press
1995, 1995
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| Edition: | 1st ed. 1995 |
| Series: | Neuromethods
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| Subjects: | |
| Online Access: | |
| Collection: | Springer Protocols Archive 1981-2004 - Collection details see MPG.ReNa |
Table of Contents:
- Technology of Patch- Clamp Electrodes
- Whole-Cell Patch-Clamp Recordings
- Pressure/Patch-Clamp Methods
- Cell-Free Ion-Channel Recording
- Perfusion of Patch Pipets
- Concentration Clamp Technique
- Perforated Patch-Clamp Technique
- The Loose Patch Voltage Clamp Technique
- Patch-Clamp Recording and RT-PCR on Single Cells
- Patch-Clamp Technique in Brain Slices
- Xenopus Oocyte Microinjection and Ion-Channel Expression