Immunocytochemical Methods and Protocols
The principle that antibodies can be used as cytochemical agents provided they are tagged with suitable markers has been evident for over 50 years. During this time the use of immunocytochemical meth ods has spread to a wide array of biological disciplines. Early applica tions focused on the detec...
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| Format: | eBook |
| Language: | English |
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Totowa, NJ
Humana Press
1995, 1995
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| Edition: | 1st ed. 1995 |
| Series: | Methods in Molecular Biology
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| Subjects: | |
| Online Access: | |
| Collection: | Springer Protocols Archive 1981-2004 - Collection details see MPG.ReNa |
Table of Contents:
- Overview of Fluorescence Photomicrography
- Overview of Fluorescence Analysis with the Confocal Microscope
- Overview of Laser Microbeam Applications as Related to Antibody Targeting
- Nucleic Acid Immunocytochemistry
- Fluorescence In Situ Hybridization Using Whole Chromosome Library Probes
- Overview of Automated Immunostainers
- Overview of Immunocytochemical Approaches to the Differential Diagnosis of Tumors
- Overview of Antibody Use in Immunocytochemistry
- Purification of Antibodies Using Ammonium Sulfate Fractionation or Gel Filtration
- Purification of Antibodies Using Ion-Exchange Chromatography
- Purification of Antibodies Using Affinity Chromatography
- Purification of Antibodies Using Protein A-Sepharose and FPLC
- Conjugation of Fluorochromes to Antibodies
- Biotinylation of Antibodies
- Overview of Cell Fixation and Permeabilization
- Preparation of Frozen Sections for Analysis
- Processing of Cell-Touch Preparations
- Processing of Cytological Specimens
- Processing of Tissue-Culture Cells
- Processing of Tissue Specimens
- Overview of Fluorophores
- Direct Immunofluorescent Labeling of Cells
- Fluorescence Labeling of Surface Antigens of Attached or Suspended Tissue-Culture Cells
- Fluorescence Labeling of Intracellular Antigens of Attached or Suspended Tissue-Culture Cells
- Fluorescent Labeling of Surface or Intracellular Antigens in Whole-Mounts
- Overview of Antigen Detection Through Enzymatic Activity
- The Peroxidase-Antiperoxidase (PAP) Method
- The Avidin-Biotin Complex(ABC) Method
- Labeled Avidin Binding (LAB) Method*
- Avidin-Biotin Labeling of Cellular Antigens in Cryostat-Sectioned Tissue
- ELISA on Attachment-Dependent or Suspension Grown Cells
- Use of Immunogold with Silver Enhancement
- Overview of Flow Cytometry and Fluorescent Probes for Cytometry
- Tissue Disaggregation
- Indirect Immunofluorescent Labeling of Viable Cells
- Indirect Immunofluorescent Labeling of Fixed Cells
- Fluorescent Labeling of DNA
- Deparaffinization and Processing of Pathologic Material
- Assay for Phagocytosis
- Assay for Filamentous Actin
- Assay for Chemoattractant Binding
- Assay for Oxidative Metabolism
- Fixation and Embedding
- Preparation of Colloidal Gold
- Conjugation of Colloidal Gold to Proteins
- Colloidal Gold/Streptavidin Methods
- Pre-Embedding Labeling Methods
- Postembedding Labeling Methods