1 “... and codon optimization of the target gene, is also a key determinant of a high-yielding protein production...”

2 Table of Contents: “... Codon Optimization On-Line (COOL) -- Shuffle Optimizer: A Program to Optimize DNA Shuffling for Protein...”

3 Table of Contents: “... and Optimization of DNA Methylation Pyrosequencing Assays Targeting Region-Specific Repeat Elements -- Determining...”

4 Table of Contents: “...Design and Testing of Regulatory Cassettes for Optimal Activity in Skeletal and Cardiac Muscle...”

5 Table of Contents: “... Pathways: Computational Sequence Design, Pathway Expression-Flux Mapping, and Scalable Pathway Optimization...”

6 Table of Contents: “... Microfluidic/Microscopy-Based Platform for On-Chip Controlled Gene Expression in Mammalian Cells -- Optimal...”

7 Table of Contents: “...Establishing an Infrastructure for High-Throughput Short-Interfering RNA Screening -- Optimization...”

8 Table of Contents: “... -- Optimized Lentiviral Transduction Protocols by use of a Poloxamer Enhancer, Spinoculation and Scfv-Antibody...”

9 Table of Contents: “...Physical Chemical and Biomolecular Methods for the Optimization of Cationic Lipid-Based Lipoplexes...”

10 “... by covering the design of DNA-binding devices, optimization of the effector domains, readout of epigenome...”

11 Table of Contents: “... -- Optimized Methodology for the Generation of RNA-sequencing Libraries from Low-input Starting Material...”

12 Table of Contents: “... in Streptomyces lividans and its Subsequent Fermentation for Optimized Production -- Degradation Network...”

13 Table of Contents: “... of Transposon Libraries -- Optimization of Synthetic Operons using Libraries of Post-Transciptional Regulatory...”

14 Table of Contents: “... -- Multiplex Real-Time PCR Using Encoded Microparticles for MicroRNA Profiling -- Optimized Whole Transcriptome...”

15 Table of Contents: “... -- Production of Fosmid Genomic Libraries Optimized for Liquid Culture Recombineering and Cross-species...”

16 Table of Contents: “... Mice -- Optimizing Particle-Mediated Epidermal Delivery of an Influenza DNA Vaccine in Ferrets...”

17 Table of Contents: “... -- The quantal nature of light -- Interaction of photons with materials -- Photoconductivity -- Photovoltaic...”

18 Table of Contents: “... Polymorphisms -- Quantifying Gene Expression in Living Cells with Ratiometric Bimolecular Beacons -- Optimizing...”

19 Table of Contents: “...Purpose and Plan -- Material, Techniques, and Preparation of the Illustrations -- Annotations...”