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130626 ||| eng |
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|a 9783540272786
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| 100 |
1 |
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|a Nölting, Bengt
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| 245 |
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|a Protein Folding Kinetics
|h Elektronische Ressource
|b Biophysical Methods
|c by Bengt Nölting
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| 250 |
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|a 2nd ed. 2006
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| 260 |
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|a Berlin, Heidelberg
|b Springer Berlin Heidelberg
|c 2006, 2006
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| 300 |
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|a XVI, 222 p. 170 illus., 12 illus. in color
|b online resource
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| 505 |
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|a Structures of proteins -- Physical interactions that determine the properties of proteins -- Calculation of the kinetic rate constants -- High kinetic resolution of protein folding events -- Kinetic methods for slow reactions -- Resolution of protein structures in solution -- High structural resolution of transient protein conformations -- Experimental problems of the kinetic and structural resolution of reactions that involve proteins -- The folding pathway of a protein (barstar) at the resolution of individual residues from microseconds to seconds -- Highly resolved folding pathways and mechanisms of six proteins -- Structural determinants of the rate of protein folding -- Evolutionary computer programming of protein structure and folding -- Conclusions
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| 653 |
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|a Protein Biochemistry
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| 653 |
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|a Food Science
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| 653 |
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|a Biotechnology
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| 653 |
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|a Biochemistry
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| 653 |
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|a Food science
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| 653 |
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|a Proteins
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| 653 |
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|a Biophysics
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| 041 |
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7 |
|a eng
|2 ISO 639-2
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| 989 |
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|b Springer
|a Springer eBooks 2005-
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| 856 |
4 |
0 |
|u https://doi.org/10.1007/b138868?nosfx=y
|x Verlag
|3 Volltext
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| 082 |
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|a 572.6
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| 520 |
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|a The book gives a deep insight into the principles and concepts of the kinetic and structural resolution of fast chemical and biophysical reactions of proteins with emphasis on protein-folding reactions. The study of fast protein-folding reactions and the understanding of the folding paradox have significantly advanced due to the recent development of new biophysical methods which allow not only kinetic resolution in the sub-millisecond time scale but also structural resolution with unprecedented precision. Pathways and structures of early and late folding events and the transition state structures of fast- and ultrafast-folding proteins can now be studied in far more detail. Important techniques include biophysical, chemical, molecular biological and mathematical methods, in particular protein engineering, Phi-value analysis, time-resolved circular dichroism, optical triggers and pulsed infrared LASER methods, pressure and temperature jump, ultrafast mixing, stopped flow and quenched flow, dielectric relaxation and electric-field-jump, acoustic relaxation, fluorescence- and isotope-labeling, H/D exchange methods, NMR line broadening and stopped-flow NMR, transition state theory, solutions of rate equations, and evolutionary computer programming. Protein Folding Kinetics - Biophysical Methods is written for students and researchers in biochemistry, biophysics, and related fields. Special features in the second edition: • Includes detailed information and 12 color figures on the high resolution of folding transition states. • Discusses structural determinants of the rate of protein folding on a timescale from microseconds to seconds. • Provides information on self-evolving computer programs for protein-folding simulations and protein-structure predictions
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