Base Excision Repair Pathway Methods and Protocols
This detailed volume provides a comprehensive set of experimental protocols and useful strategies to examine the repair of damaged bases via the Base Excision Repair (BER) pathway in vitro and in cells. Beginning with multiple molecular and cellular techniques to examine the excision of damaged base...
| Other Authors: | , |
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| Format: | eBook |
| Language: | English |
| Published: |
New York, NY
Humana
2023, 2023
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| Edition: | 1st ed. 2023 |
| Series: | Methods in Molecular Biology
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| Subjects: | |
| Online Access: | |
| Collection: | Springer eBooks 2005- - Collection details see MPG.ReNa |
Table of Contents:
- Simultaneous Short and Long Patch Base Excision Repair (BER) Assay in Live Mammalian Cells
- In Vitro Assay to Measure APE1 Enzymatic Activity on Ribose Monophosphate Abasic Site
- Highly Sensitive Radioactivity-Based DNA 3’-Phosphatase Activity Assay for Polynucleotide Kinase 3’-Phosphatase
- Generation of Recombinant Nucleosomes Containing Site-Specific DNA Damage
- A DNA Cleavage Assay Using Synthetic Oligonucleotide Containing a Single Site-Directed Lesion for In Vitro Base Excision Repair Study
- In Vitro Reconstitutive Base Excision Repair (BER) Assay
- Detection of Oxidatively Modified Base Lesion(s) in Defined DNA Sequences by FLARE Quantitative PCR
- Isolation and Immunodetection of Enzymatic DNA-Protein Crosslinks by RADAR Assay
- Slot Blot Assay for Detection of R Loops
- Assays with Patient-Derived Organoids to Evaluate the Impact of Microbial Infection on the Base Excision Repair (BER) Enzymes
- Characterizing the Repair of DNA Double-Strand Breaks: A Review of Surrogate Plasmid-Based Reporter Methods
- Interactome Profiling of DNA Damage Response (DDR) Mediators with Immunoprecipitation-Mass Spectrometry
- Using Affinity Pulldown Assays to Study Protein-Protein Interactions of Human NEIL1 Glycosylase and the Checkpoint Protein RAD9-RAD1-HUS1 (9-1-1) Complex
- Tandem Affinity Purification and Mass-Spectrometric Analysis of FACT and Associated Proteins
- Analysis of Copy Number Variation of DNA Repair/Damage Response Genes in Tumor Tissues
- Genome-Wide Binding Analysis of DNA Repair Protein APE1 in Tumor Cells by ChIP-Seq
- Tumorsphere Formation Assay: A Cancer Stem-Like Cell Characterization in Pediatric Brain Cancer Medulloblastoma