Nipah Virus Methods and Protocols

This volume summarizes key methods and advancements in the field of henipavirus molecular biology. Chapters guide readers through protocols focusing on emerging bat-borne paramyxoviruses as well as pathogenicity caused by Nipah and Hendra viruses. Protocols are also included to help develop diagnost...

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Bibliographic Details
Other Authors: Freiberg, Alexander N. (Editor), Rockx, Barry (Editor)
Format: eBook
Language:English
Published: New York, NY Humana 2023, 2023
Edition:1st ed. 2023
Series:Methods in Molecular Biology
Subjects:
Online Access:
Collection: Springer eBooks 2005- - Collection details see MPG.ReNa
Table of Contents:
  • Overview of experimental vaccines and antiviral therapeutics for Henipavirus infection.-A Revised Diagnostic Quantitative RT-PCR for Detection of Nipah Virus Infection
  • Recombinant Soluble Henipavirus Glycoprotein Preparation
  • Cell-cell fusion assays to study henipavirus entry and evaluate therapeutics
  • Recombinant Cedar virus: a henipavirus reverse genetics platform
  • Utilizing Recombinant Reporter Henipaviruses to Conduct Antiviral Screening
  • In vitro antiviral screening for henipaviruses at BSL4
  • Isolation of primary porcine bronchial epithelial cells for Nipah virus infections
  • Primary culture of the human olfactory neuroepithelium and utilization for henipavirus infection in vitro
  • Mouse models of Henipavirus infection
  • In vivo imaging of Nipah virus infection in small animal rodent models
  • Nonhuman Primate Models for Nipah and Hendra Virus Countermeasure Evaluation
  • Nipah virus aerosol challenge of three distinct particle sizes in nonhuman primates
  • Generation and characterization of a humanized lung xenograft mouse model for studying henipavirus pathogenesis
  • Ferret models for henipavirus infection
  • Syrian golden hamster model for Nipah virus infection
  • Anti-Nipah Virus Enzyme-Linked Immunosorbent Assays with Nonhuman Primate and Hamster Serum
  • Assays for Detecting Henipavirus Antibodies
  • Profiling host microRNA responses to henipavirus infection
  • Host Transcriptome Analysis of Ferret Tissues Following Henipavirus Infection.