Difference Gel Electrophoresis Methods and Protocols
The second edition of this volume provides a comprehensive update of this key method on gel-based proteomics. Chapters present an introduction into the development of methods on principles of differential protein labeling and two-dimensional gel electrophoresis, techniques on optimized proteomic wor...
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| Format: | eBook |
| Language: | English |
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New York, NY
Humana
2023, 2023
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| Edition: | 2nd ed. 2023 |
| Series: | Methods in Molecular Biology
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| Subjects: | |
| Online Access: | |
| Collection: | Springer eBooks 2005- - Collection details see MPG.ReNa |
Table of Contents:
- Two-Dimensional Gel Electrophoresis and 2D-DIGE
- Top-down Proteomics and Comparative 2D-DIGE Analysis
- DIGE Analysis Software and Protein Identification Approaches
- Native DIGE for Quantitative and Functional Analysis of Protein Interactomes
- Comparative Two-dimensional Fluorescence Gel Electrophoresis
- Identification of Ubiquitination associated Proteins using 2D-DIGE
- DIGE-based Phosphoproteomic Analysis
- DIGE-based Biomarker Discovery in Blood Cancers
- DIGE Saturation Labeling for Scarce Amounts of Protein from Formalin-fixed Paraffin-embedded (FFPE) Tissue
- DIGE Analysis of ProteoMiner™ Fractionated Serum/Plasma Samples
- Comparative 3-sample 2D-DIGE Analysis of Skeletal Muscles
- Proteomic identification of saliva proteins as non-invasive diagnostic biomarkers
- Analysis of the saliva proteome using 2D-DIGE
- DIGE Analysis of Clinical Specimens
- DIGE Analysis of Animal Tissues
- Rapid 2D-DIGE Proteomic Analysis of Mouse Liver within One Week
- 2D-DIGE analysis of liver disease in mice
- 2-Dye Versus 3-Dye DIGE for Comparative Testis Tissue Proteomic Analysis
- 2-CyDye based 2D-DIGE Analysis of Aged Human Muscle Biopsy Specimens
- Identification of Subproteomic Markers for Skeletal Muscle Profiling
- DIGE Analysis of Fish Tissues
- Sample preparation and protein determination for 2D-DIGE proteomics
- Protein Digestion for 2D-DIGE Analysis
- Subcellular Fractionation for DIGE-based Proteomics
- DIGE Analysis of Immunodepleted Plasma
- Bioinformatic Analysis of the Subproteomic Profile of Cardiomyopathic Tissue
- Elucidating Cellular Metabolism and Protein Difference Data from DIGE Proteomics Experiments using Enzyme Assays
- Enzyme Assay Methods to Validate DIGE Proteomics Data
- Immunoblot Analysis of DIGE-based Proteomics
- Verification of Protein Changes determined by 2D-DIGE based Proteomics using Immunofluorescence Microscopy
- Histological and Histochemical Microscopy used to verify 2D-DIGE Pathoproteomics.