Chromaffin Cells Methods and Protocols
This volume covers the most up-to-date methods and techniques used to further the understanding of chromaffin cell biology and pharmacology. Chapters guide readers through the basic mechanisms that regulate the stimulus-secretion coupling, chromaffin, tumor-derived cell PC-12 , morphology, biochemis...
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| Format: | eBook |
| Language: | English |
| Published: |
New York, NY
Humana
2023, 2023
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| Edition: | 1st ed. 2023 |
| Series: | Methods in Molecular Biology
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| Subjects: | |
| Online Access: | |
| Collection: | Springer eBooks 2005- - Collection details see MPG.ReNa |
Table of Contents:
- Rat pheochromocytoma PC12 cells in culture
- Adrenal grafts in the central nervous system: Chromaffin and chromaffin progenitor cell transplantation
- Immunocytochemistry of acutely isolated adrenal medullary chromaffin cells
- Transmission electron microscopy: a method for studying the adrenochromaffin cells
- Immunogold for protein location in chromaffin cells
- Visualization of exo- and endocytosis membrane dynamics with super-resolution STED microscopy
- Online detection of catecholamine release from the perfused rat adrenal gland
- On-line recording of perifused chromaffin cells
- Recording of chromaffin cell electrical activity in situ in acute adrenal slices
- Calcium imaging and amperometric recording in cultured chromaffin cells and adrenal slices from normotensive, Wistar Kyoto rats, and Spontaneously Hypertensive Rats
- Measurements of calcium in chromaffin cell organelles using targeted aequorins
- Quantification of secretory granule exocytosis by TIRF imaging and capacitance measurements
- Membrane capacitance measurements of stimulus-evoked exocytosis in adrenal chromaffin cell
- Quantal release analysis of electrochemically active molecules using single-cell amperometry
- Methodologies for detecting quantal exocytosis in adrenal chromaffin cells through diamond-based MEAs
- Vesicle collision protocols for the study of quantum size and exocytotic fraction released
- Patch Amperometry and Intracellular Patch Electrochemistry
- Artificial cells for dissecting exocytosis
- Isolation and purification of chromaffin granules from adrenal glands and cultured neuroendocrine cells
- Confocal microscopy studies of F-actin cytoskeleton distribution and dynamics using fluorescent LifeAct constructs in bovine adrenal chromaffin cells
- Unveiling the nanoscale dynamics of the exocytic machinery in chromaffin cells with single-molecule imaging
- Determinationof catecholamines in a small volume (25µl) of plasma from conscious mouse tail vein
- Quantification of chromogranin A and its fragments in biological fluids
- Cytotoxicity models in chromaffin cells to evaluate neuroprotective compounds