Regulatory T-Cells Methods and Protocols
This volume provides methods and models for investigating the immunosuppressive subset of CD4+ T-cells, regulatory T-cells (Treg). Chapters guide readers through, basic protocols for analyzing Treg in mice and humans, single-cell analysis for analyzing Treg, and methods for the analysis of Treg in c...
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| Format: | eBook |
| Language: | English |
| Published: |
New York, NY
Humana
2023, 2023
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| Edition: | 1st ed. 2023 |
| Series: | Methods in Molecular Biology
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| Subjects: | |
| Online Access: | |
| Collection: | Springer eBooks 2005- - Collection details see MPG.ReNa |
Table of Contents:
- Analysis of the in vivo function of follicular regulatory T (TFR) cells in the regulation of antibody response
- Analysis of peripherally derived Treg in the intestines
- Isolation and analysis of tumor infiltrating Treg
- Induction of peripheral Treg
- Adoptive transfer and bone marrow chimera models to analyse Treg function and differentiation
- Functional analysis of Foxp3 and its mutants by retroviral transduction of murine primary CD4+ T cells
- Genetic Tools for Analyzing Foxp3+ Treg cells: Fluorochrome-Based Transcriptional Reporters and Genetic Fate-Mapping
- Depletion of Treg by the Diphtheria Toxin System
- Analysis of FOXP3 DNA methylation patterns to identify functional FOXP3+ T-Cell subpopulations
- Protein Ubiquitination Assay
- Phenotypic and Functional Studies of Human Treg cell Subpopulations
- In vitro analysis of CTLA-4 mediated transendocytosis by Regulatory T cells
- Isolation and functional characterisation of regulatory CD4+ Tcells from the inflamed joints of patients with rheumatoid arthritis
- A GMP protocol for the manufacture of regulatory T cells for clinical application
- Regulatory T-cell phenotyping using CyTOF
- High-dimensional single-cell profiling of tumor-infiltrating CD4+ regulatory T cells
- Single-cell transcriptome analysis of Treg