Candida Species Methods and Protocols

This updated volume explores Candida and candidiasis methods, useful to a wide variety of Candida scientists including those new to the field. Beginning with a section on the Candida genome, the book continues by covering techniques for antifungal discovery and studying resistance, pathogenesis and...

Full description

Bibliographic Details
Other Authors: Calderone, Richard (Editor)
Format: eBook
Language:English
Published: New York, NY Humana 2022, 2022
Edition:2nd ed. 2022
Series:Methods in Molecular Biology
Subjects:
Online Access:
Collection: Springer eBooks 2005- - Collection details see MPG.ReNa
Table of Contents:
  • CRISPR-Mediated Genome Editing in the Human Fungal Pathogen C. albicans
  • Plasmid-Based CRISPR–Cas9 Editing in Multiple Candida Species
  • A Proteomic Approach for the Quantification of Post-Translational Protein Lysine Acetylation in Candida albicans
  • How to Use the Candida Genome Database
  • Sub-Telomeric Chromatin Structure by Chromosome Conformation Capture (3C)-qPCR Methodology in Candida glabrata
  • Construction of Double Heterozygous Deletion Strains for Complex Haploinsufficiency-Based Genetic Analysis in Candida albicans
  • Using Synthetic Genetic Interactions in Candida glabrata as a Novel Method to Detect Genes with Roles in Antifungal Drug Resistance
  • Identifying Specific Small Molecule-Protein Interactions Using Target Abundance-Based Fitness Screening (TAFiS)
  • High Throughput Phospholipidomics of Candida Cells: From Sample Preparation to Data Analysis
  • Characterization of the Effects of Candida Gastrointestinal Colonization on Clostridioides difficile Infection in a Murine Model
  • Analysis of Gut Microbiome Using Fecal Samples
  • Measurements of Treg Cell Induction by Candida albicans DNA Using Flow Cytometry
  • Molecular and Microscopic Methods of Quantifying Candida albicans Cell Wall PAMP Exposure
  • Isolation, Physicochemical Characterization, Labeling, and Biological Evaluation of Mannans and Glucans
  • Understanding the Protective Role of IL-17 during Oropharyngeal Candidiasis
  • Genetic Screens of an Anti-Candida Natural Product Using the Heterozygous Saccharomyces cerevisiae Mutant Library
  • Assessing Complex I (CI) Mitochondrial Subunit Protein Functions in Candida albicans
  • In Vitro Biophysical Characterization of Candidalysin: A Fungal Peptide Toxin
  • Assessment of cAMP-PKA Signaling in Candida glabrata by FRET-Based Biosensors
  • Methods Related to the Immunopathogenesis of Vulvovaginal Candidiasis and Associated Neutrophil Anergy
  • A Simple Method for Growth of Candida albicans Biofilms under Continuous Media Flow and for Recovery of Biofilm Dispersed Cells
  • A Simple 96 Well Plate-Based Method for Development of Candida Biofilms under Static Conditions
  • Comparative Analysis of the Fitness of Candida albicans Strains during Colonization of the Mice Gastrointestinal Tract
  • Detection and Identification of Candida auris from Clinical Skin Swabs
  • Production and Isolation of the Candida Species Biofilm Extracellular Matrix