Immunohistochemistry and Immunocytochemistry Methods and Protocols
This volume provides a comprehensive reference guide for researchers to study the applications of labeled antibodies. Chapters guide reader through the the and practice of immunohistochemistry, immunocytochemistry and immunofluorescence techniques. Written in the highly successful Methods in Molecul...
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| Format: | eBook |
| Language: | English |
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New York, NY
Humana
2022, 2022
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| Edition: | 1st ed. 2022 |
| Series: | Methods in Molecular Biology
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| Subjects: | |
| Online Access: | |
| Collection: | Springer eBooks 2005- - Collection details see MPG.ReNa |
Table of Contents:
- Introduction to Immunohistochemistry. From Evolving Science to Timeless Art
- Immunohistochemistry in Historical Perspective. Knowing the Past to Understand the Present
- Sample Preparation & Fixation for Histology and Pathology
- Tissue Processing
- Antigen Retrieval and Signal Amplification
- Primary Antibody Selection and Blocking Techniques for Immunohistochemistry
- Probes for Fluorescent Visualization of Specific Cellular Organelles
- Enzymatic Immunohistochemistry
- Fluorescent Immunohistochemistry
- Double Labeling Fluorescent Immunocytochemistry
- Multiplexing and Spectral Microscopy
- In situ Hybridization (Fluorescent and Enzymatic)
- Proximity Ligation Assay (PLA)
- Immunohistochemistry, Quality control and Principles of Validation in the Central Nervous System
- Culture and Phenotyping of Glial Cell Cultures, Gliospheres and Neurospheres
- ER+ Breast Cancer Mammosphere Formation and Analysis
- Detection of Circulating and Tissue Myeloid-Derived Suppressor Cells (MDSCs) by Flow Cytometry
- Comet Assay for the Detection of Single and Double Strand DNA Breaks
- Fluorescent-Activated Cell Sorting (Flow Cytometry)