Rice Genome Engineering and Gene Editing Methods and Protocols

This detailed volume explores rice molecular biology, genetic engineering, and genome editing technologies. Dividing into three parts, the book covers subjects such as genetic engineering and tissue culture of rice, including efficient methods for rice transformation and regeneration, genome editing...

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Bibliographic Details
Other Authors: Bandyopadhyay, Anindya (Editor), Thilmony, Roger (Editor)
Format: eBook
Language:English
Published: New York, NY Springer US 2021, 2021
Edition:1st ed. 2021
Series:Methods in Molecular Biology
Subjects:
Online Access:
Collection: Springer eBooks 2005- - Collection details see MPG.ReNa
Table of Contents:
  • Identification and Analysis of Steroidogenic Acute Regulatory (StAR) Protein-Related Lipid Transfer (START) Domains in Rice
  • Assessing Rice Salinity Tolerance: From Phenomics to Association Mapping
  • Gene Assembly in Agrobacterium via Nucleic Acid Transfer Using Recombinase Technology (GAANTRY)
  • TransGene Stacking II Vector System for Plant Metabolic Engineering and Synthetic Biology
  • Morphogenic Regulators and Their Application in Improving Plant Transformation
  • A Rapid Method for Stably Transforming Rice Seeds
  • Agrobacterium tumefaciens-Mediated Transformation of Rice by Hygromycin Phosphotransferase (hptII) Gene Containing CRISPR/Cas9 Vector
  • Genotype Independent Regeneration and Transformation Protocol for Rice Cultivars
  • Rapid Vector Construction and Assessment of BE3 and Target-AID C to T Base Editing Systems in Rice Protoplasts
  • Genome Editing of Rice by CRISPR-Cas: End-to-End Pipeline for Crop Improvement
  • Single Base Editing Using Cytidine Deaminase to Change Grain Size and Seed Coat Color in Rice
  • Analysis of Off-Target Mutations in CRISPR-Edited Rice Plants Using Whole Genome Sequencing
  • Efficient Genome Editing in Rice Protoplasts Using CRISPR/CAS9 Construct
  • Single Transcript Unit CRISPR 2.0 Systems for Genome Editing in Rice
  • Improving a Quantitative Trait in Rice by Multigene Editing with CRISPR-Cas9
  • Rice Haploid Inducer Development by Genome Editing
  • FLP-Mediated Site-Specific Gene Integration in Rice
  • Rice Gene Targeting by Homologous Recombination with Positive Negative Selection Strategy
  • Prediction of Rice Transcription Start Sites Using TransPrise: A Novel Machine Learning Approach
  • Single Cell Type Specific RNA Isolation and Gene Expression Analysis in Rice Using Laser Capture Microdissection (LCM)-Based Method
  • Immunolocalization Analysis of C4 Proteins in the Leaf Tissue of Rice
  • Selection of Suitable Reference Genes for qRT-PCR Gene Expression Studies in Rice
  • Rice Protoplast Isolation and Transfection for Transient Gene Expression Analysis