Testicular Germ Cell Tumors Methods and Protocols

This volume presents the latest techniques used to study germ cell tumors (GCTs), including histopathology; in vitro and in vivo techniques for investigating GCT biology; novel methods for detecting minimal residual diseases; and sequencing considerations as they apply to GCTs. Chapters cover topics...

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Bibliographic Details
Other Authors: Bagrodia, Aditya (Editor), Amatruda, James F. (Editor)
Format: eBook
Language:English
Published: New York, NY Humana 2021, 2021
Edition:1st ed. 2021
Series:Methods in Molecular Biology
Subjects:
Online Access:
Collection: Springer eBooks 2005- - Collection details see MPG.ReNa
Table of Contents:
  • Basic Histopathologic Assessment of Germ Cell Tumors for Clinic and Research
  • Clinical Applications of Immunohistochemistry in Germ Cell Tumors in Men
  • Molecular Characterization of Testicular Germ Cell Tumors Using Tissue Microdissection
  • Fluorescence In Situ Hybridization (FISH) Detection of Chromosomal 12p Anomalies in Testicular Germ Cell Tumors
  • Germ Cell Tumor Cell Culture Techniques
  • Three-Dimensional Cultivation of Germ Cell Cancer Cell Lines as Hanging Drops
  • Cultivation of Testicular Germ Cell Cancer Cell Lines and Establishment of Gene-Edited Subclones using CRISPR/Cas9
  • Evaluation of Chemotherapeutic Drugs for Treatment of (Cisplatin-Resistant) Germ Cell Cancer Cell Lines
  • Assessing Homologous Recombination and Interstrand Crosslink Repair in Embryonal Carcinoma Testicular Germ Cell Tumor Cell Lines
  • Production and Analysis of Human Primordial Germ Cell-Like Cells
  • A Genetically Engineered Mouse Model of Malignant Testicular Germ Cell Tumors
  • Targeted Methylation Analyses: From Bisulfite Treatment to Quantification
  • Integrated Analysis of Germ Cell Tumors
  • Use of Genome-Wide Association Studies to Evaluate Genetic Predisposition to Testicular Germ Cell Tumors
  • A Circulating MicroRNA Panel for Malignant Germ Cell Tumor Diagnosis and Monitoring
  • Detection of Circulating Tumor Cells (CTCs) in Patients with Testicular Germ Cell Tumors
  • Developing and Using a Data Commons for Understanding the Molecular Characteristics of Germ Cell Tumors