Downstream Processing of Proteins Methods and Protocols
In Downstream Processing of Proteins: Methods and Protocols, Mohamed A. Desai and a team of experienced biotechnologists review both conventional and novel isolation techniques used in industrial applications for the downstream processing of protein molecules. These techniques include primary and se...
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| Format: | eBook |
| Language: | English |
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Totowa, NJ
Humana
2000, 2000
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| Edition: | 1st ed. 2000 |
| Series: | Methods in Biotechnology
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| Collection: | Springer Book Archives -2004 - Collection details see MPG.ReNa |
Table of Contents:
- Downstream Processing in the Biotechnology Industry
- 2 Microbial Cell Disruption by High-Pressure Homogenization
- 3 Protein Concentration and Buffer Exchange Using Ultrafiltration
- 4 High-Performance Tangential Flow Filtration for Protein Separations
- Large-Scale Recovery of Protein Inclusion Bodies by Continuous Centrifugation
- Application of Density Gradient Ultracentrifugation Using Zonal Rotors in the Large-Scale Purification of Biomolecules
- Application of Chromatography in the Downstream Processing of Biomolecules
- Affinity Ligand Technology
- Immunoaffinity Adsorption in the Large-Scale Isolation of Biomolecules
- Expanded Bed Adsorption in the Purification of Biomolecules
- Application of HPLC in the Purification of Biomolecules
- Countercurrent Chromatography of Proteins with Polymer Phase Systems
- Practical Aspects and Applications of Radial Flow Chromatography
- Sterile Filtration and Virus Filtration
- Design and Interpretation of Viral Clearance Studies for Biopharmaceutical Products
- Validation of Sterilizing Filters in the Biotechnology Industry