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200811 ||| eng |
| 020 |
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|a 9781592590766
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| 100 |
1 |
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|a George, Andrew J.T.
|e [editor]
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| 245 |
0 |
0 |
|a Diagnostic and Therapeutic Antibodies
|h Elektronische Ressource
|c edited by Andrew J.T. George, Catherine E. Urch
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| 250 |
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|a 1st ed. 2000
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| 260 |
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|a Totowa, NJ
|b Humana
|c 2000, 2000
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| 300 |
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|a XIV, 477 p
|b online resource
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| 505 |
0 |
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|a The Antibody Molecule -- Polyclonal and Monoclonal Antibodies -- Engineering Antibody Molecules -- Phage Display Technology -- Antibodies in Medicine -- Prospects for the Application of Antibodies in Medicine -- Antibodies for Neoplastic Disease -- The Application of Monoclonal Antibodies in the Treatment of Lymphoma -- Antibodies for Inflammatory Disease -- Antibodies for Inflammatory Disease -- Antibodies for Transplantation -- Antibody-Based Therapies in Infectious Diseases -- Antibodies in Nuclear Medicine -- Animal Models for Tumor Localization -- Antibodies for Immunoassays -- Ethics and Industry -- Intellectual Property -- From Laboratory to Clinic -- Production and Purification -- Production of Monoclonal Antibodies -- Purification of Monoclonal Antibodies Using Protein A/G -- Preparation of Monoclonal Antibodies Using Ion Exchange Chromatography -- Quality Control of Raw Materials -- Cell Banks and Stability of Antibody Production -- Measurement of Endotoxin -- Aseptic Vial Filling -- Measurement of Antibody Concentrations by Hemagglutination -- Modification of Antibodies -- Enzymatic Digestion of Monoclonal Antibodies -- How to Make Bispecific Antibodies -- Radiolabeling of Monoclonal Antibodies -- Determination of the Immunoreactivity of Radiolabeled Monoclonal Antibodies -- Use of Biosensors to Measure the Kinetics of Antibody-Antigen Interactions -- Application of Antibodies in Vitro -- How to Set Up an ELISA -- Measurement of HAMA and Anti-Idiotypic Antibodies -- SDS-PAGE and Western Blotting -- Flow Cytometric Analysis -- Immunocytochemistry -- Immunolabeling for Electron Microscopy -- Antibody Engineering -- PCR of the V Region -- Phage Display Technology
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| 653 |
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|a Immunology
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| 700 |
1 |
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|a Urch, Catherine E.
|e [editor]
|
| 041 |
0 |
7 |
|a eng
|2 ISO 639-2
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| 989 |
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|b SBA
|a Springer Book Archives -2004
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| 490 |
0 |
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|a Methods in Molecular Medicine
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| 028 |
5 |
0 |
|a 10.1385/1592590764
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| 856 |
4 |
0 |
|u https://doi.org/10.1385/1592590764?nosfx=y
|x Verlag
|3 Volltext
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| 082 |
0 |
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|a 616.079
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| 082 |
0 |
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|a 571.96
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| 520 |
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|a The use of monoclonal antibodies in treating disease has long held tantalizing promise, and the recent explosion of successful clinical trials-leading to the licensing of novel antibody-based therapeutic agents-has clearly demonstrated the power of recombinant antibody engineering, and greatly rejuvenated interest in their emergent clinical applications. In Diagnostic and Therapeutic Antibodies, a team of experts comprehensively reviews the theoretical and practical aspects of applying antibodies in both the laboratory and clinic. For general understanding, the book thoroughly introduces the basic science of the antibody molecule, including its structure and generation. Several novel in vivo applications of therapeutic antibodies are then presented to illustrate their special value in varied clinical settings. For those new to the field, there are readily reproducible methods for generating and purifying antibodies and for modifying them for clinical application, including how to measure their affinity and immunoactivity, and how to employ them in staining procedures and in a variety of in vitro assays. Timely and comprehensive, Diagnostic and Therapeutic Antibodies offers today‚s clinical researchers a concise introduction to the field, as well as a highly useful compendium of practical protocols that will greatly facilitate the application of these powerful new diagnostic and therapeutic agents
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