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Characterization of Plant Viruses Methods and Protocols

This book provides detailed information on methodologies used in biological, serological and nucleic acid based assays for the detection, diagnosis and management of plant viruses. The content is divided into six main parts, the first of which presents techniques used in the biological characterizat...

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Bibliographic Details
Main Authors: Bhat, Alangar Ishwara, Rao, Govind Pratap (Author)
Format: eBook
Language:English
Published: New York, NY Humana 2020, 2020
Edition:1st ed. 2020
Series:Springer Protocols Handbooks
Subjects:
Virology
Plant Diseases
Plant Development
Plant Pathology
Plants / Development
Plant Genetics
Plant Physiology
Online Access:
Collection: Springer eBooks 2005- - Collection details see MPG.ReNa
Table of Contents:
  • Chapter 1. Glasshouse for maintenance of virus and insect culture
  • Chapter 2. Symptoms of virus infected plants
  • Chapter 3. Isolation and diagnosis of virus through indicator hosts
  • Chapter 4. Host range of viruses
  • Chapter 5. Physico-chemical properties of virus in crude sap
  • Chapter 6. Mechanical sap transmission
  • Chapter 7. Transmission through grafting and budding
  • Chapter 8. Transmission through dodder
  • Chapter 9. Virus transmission through Pollen
  • Chapter 10. Transmission through seeds
  • Chapter 11. Transmission of viruses by aphids
  • Chapter 12. Transmission of viruses by leafhoppers
  • Chapter 13. Transmission of virusesby whiteflies
  • Chapter 14. Transmission of virusesby thrips
  • Chapter 15. Transmission of viruses through mealybugs
  • Chapter 16. Transmission of viruses through beetles
  • Chapter 17. Transmission of viruses through mites
  • Chapter 18. Transmission of viruses through fungi
  • Chapter 19. Transmission of viruses through nematodes
  • Chapter 36. Real-time polymerase chain reaction
  • Chapter 37. DNA Microarray for detection of plant viruses
  • Chapter 38. Loop-mediated isothermal amplification (LAMP)
  • Chapter 39. Rolling circle amplification (RCA)
  • Chapter 40. Recombinase polymerase amplification
  • Chapter 41. Next generation sequencing for diagnosis of viruses
  • Chapter 42. Cloning of PCR Product
  • Chapter 43. cDNA synthesis and cloning
  • Chapter 44. DNA sequencing
  • Chapter 45. Sequence analysis and phylogenetic studies
  • Chapter 46. Development of infectious clone of virus
  • Chapter 47. Virus elimination by meristem-tip culture
  • Chapter 48. Virus elimination through somatic embryogenesis
  • Chapter 49. Production of virus-resistant plants through transgenic approaches
  • Chapter 50. Production of virus-resistant plants through CRISPR-Cas technology
  • Chapter 20. Storage andpreservation of plant virus cultures
  • Chapter 21. Purification of Plant Viruses
  • Chapter 22. Ultraviolet absorption spectra of purified virus preparation
  • Chapter 23. Electron microscopy and Utramicrotomy
  • Chapter 24. Determination of coat protein molecular weight of viruses
  • Chapter 25. Isolation of nucleic acid from purified virus and determination of its nature
  • Chapter 26. Agarose gel electrophoresis for nucleic acids
  • chapter 27. In vitro expression of viral coat protein in prokaryotic system and its purification
  • Chapter 28. Production of Polyclonal Antiserum
  • Chapter 29. Production of monoclonal antibody
  • chapter 30. Serological Tests
  • Chapter 31. Isolation of total DNA from plants
  • Chapter 32. Isolation of total RNA from plants
  • Chapter 33. Isolation of double stranded (ds) RNA from virus infected plants
  • Chapter 34. Dot-blot hybridization technique
  • Chapter 35. Polymerase chain reaction

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