RNA Chaperones Methods and Protocols
This book provides a wide spectrum of methods to study RNA chaperones in vitro, at the single molecule level, and protocols useful for cell-based assays. Beginning with a section on a number of bacterial proteins for study, the volume also explores proteins from eukaryotic cells and how to delve int...
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| Format: | eBook |
| Language: | English |
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New York, NY
Humana
2020, 2020
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| Edition: | 1st ed. 2020 |
| Series: | Methods in Molecular Biology
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| Subjects: | |
| Online Access: | |
| Collection: | Springer eBooks 2005- - Collection details see MPG.ReNa |
Table of Contents:
- Biochemical Methods for the Study of the FinO Family of Bacterial RNA Chaperones
- Quantitative Analysis of RNA Chaperone Activity by Native Gel Electrophoresis and Fluorescence Spectroscopy
- Fluorescent Molecular Beacons Mimicking RNA Secondary Structures to Study RNA Chaperone Activity
- Specific Nucleic Acid Chaperone Activity of HIV-1 Nucleocapsid Protein Deduced from Hairpin Unfolding
- Real-Time Fluorescence-Based Approaches to Disentangle Mechanisms of a Protein’s RNA Chaperone Activity
- Use of tRNA-Mediated Suppression to Assess RNA Chaperone Function
- Salt-Dependent Modulation of the RNA Chaperone Activity of RNA-Binding Protein La
- Kinetic and Thermodynamic Analyses of RNA–Protein Interactions
- Detection of MicroRNAs Released from Argonautes
- Mapping the RNA Chaperone Activity of the T. brucei Editosome Using SHAPE-Chemical Probing
- RNA Remodeling by RNA Chaperones Monitored by RNA Structure Probing
- In Vivo RNA Chemical Footprinting Analysis in Archaea
- RNA Structure Analysis by Chemical Probing with DMS and CMCT
- Disordered RNA Binding Region Prediction with DisoRDPbind
- Delivering Molecular Beacons via an Electroporation-Based Approach Enables Live-Cell Imaging of Single RNA Transcripts and Genomic Loci
- Site-Specific Dual-Color Labeling of Long RNAs
- Single-Molecule FRET Assay for Studying Cotranscriptional RNA Folding
- Characterizing Complex Nucleic Acid Interactions of LINE1 ORF1p by Single Molecule Force Spectroscopy
- Isolation and Analysis of Bacterial Ribosomes through Sucrose Gradient Ultracentrifugation