DNA Sequencing Protocols

The purpose of DNA Sequencing Protocols is to provide detailed practical procedures for the widest range of DNA sequencing meth­ ods, and we believe that all the vanguard techniques now being applied in this fast-evolving field are comprehensively covered. Sequencing technology has advanced at a phe...

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Main Authors: Griffin, Annette M., Griffin, Hugh G. (Author)
Corporate Author: SpringerLink (Online service)
Format: eBook
Published: Totowa, NJ Humana Press 1993, 1993
Edition:1st ed. 1993
Series:Methods in Molecular Biology
Online Access:
Collection: Springer Book Archives -2004 - Collection details see MPG.ReNa
Table of Contents:
  • DNA Sequencing
  • M13 Cloning Vehicles
  • Cloning into M13
  • Transfection of E. coli with M13 DNA
  • M13 Phage Growth and Single-Strand DNA Preparation
  • M13 Phage Growth and DNA Purification Using 96 Well Microtiter Trays
  • Generation of Random Fragments by Sonication
  • Generation of a Nested Set of Deletions Using Exonuclease III
  • Sequential Deletions of Single-Stranded DNA
  • Subcloning for DNA Sequencing
  • Sequencing Using Custom Designed Oligonucleotides
  • Dideoxy Sequencing Reactions Using Klenow Fragment DNA Polymerase 1
  • Dideoxy Sequencing Reactions Using T7 Polymerase
  • Dideoxy Sequencing Reactions Using Sequenase Version 2.0
  • Dideoxy Sequencing Reactions Using Taq Polymerase
  • Pouring Linear and Buffer-Gradient Sequencing Gels
  • Electrophoresis of Sequence Reaction Samples
  • Plasmid Sequencing
  • Plasmid Sequencing
  • Direct Sequencing of Inserts Cloned into Lambda Vectors
  • Cosmid Sequencing
  • Genomic Sequencing
  • Sequencing of Double-Stranded PCR Produ