Hepatitis C Protocols
In Hepatitis C Protocols, Johnson YN Lau and his expert collaborators present cutting-edge techniques for study of the hepatitis C virus infection and its disease, hepatitis C, which afflict an estimated 170 million people worldwide. The methods range from the detection of anti-HCV in serum and HCV...
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| Format: | eBook |
| Language: | English |
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Totowa, NJ
Humana
1998, 1998
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| Edition: | 1st ed. 1998 |
| Series: | Methods in Molecular Medicine
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| Subjects: | |
| Online Access: | |
| Collection: | Springer Book Archives -2004 - Collection details see MPG.ReNa |
Table of Contents:
- Production of Replication-Deficient Adenovirus Recombinants
- Generation of Recombinant Herpes Simplex Virus Ampiicons
- Construction of Recombinant Sindbis-Based Expression Vectors for the Study of HCV Genes and Their Products
- In Vivo Models
- The Chimpanzee Model
- Liver Transplantation as a Model to Study Hepatitis C Virus Infection
- Use of the Vaccinia Virus/T7 Expression System for Studying HCV Protein Processing
- Use of a Discistronic Vector for the Quantitation of HCV IRES Activity
- Expression and Dimerization of Hepatitis C Virus Core Protein in E. coli
- Expression and Characterization of the HCV NS2 Protease
- Expression and Characterization of HCV NS3 Protease
- Expression and Characterization of the HCV NS3 Helicase Domain
- Hepatitis C Virus RNA-Dependent RNA Polymerase (NS5B Polymerase)
- Detection and Molecular Cloning of the Extreme 3?-End of HCV
- In Vitro Stability of Hepatitis C Virus RNA
- Methods for the Study of Sequence-Specific Binding of Proteins to the HCV RNA Genome
- HCV-Specific Immunologic Response
- Role of Immune Response in HCV
- Determination of Hepatitis C Virus-Specific CD4+ T-Cell Activity in PBMC
- Measurement of HCV-Specific CD8+ Cytotoxic T-Cell Activities in the Peripheral Blood by Europium Release Assay
- Detection of Anti-HCV in Serum
- Detection of Anti-GOR Antibodies in HCV Infection
- Confirmation of HCV Antibodies by the Line Immunoassay INNO-LIA HCV Ab III
- Detection and Quantitation of HCV in Serum
- Detection of HCV RNA in Serum by Reverse Transcriptase-PCR and Radiolabeled Liquid Hybridization
- Detection of HCV RNA in Serum by Reverse Transcription Polymerase Chain Reaction (RT-PCR)
- The AMPLICOR® HCV Tests for the Detection and Quantitation of Serum or Plasma HCV RNA
- Quantification of HCV RNA in Clinical Specimens by Branched DNA (bDNA) Technology
- Quality Control of the Polymerase Chain Reaction
- Preparation of Genotype-Specific HCV RNA Transcripts for Assessing HCV Detection and Quantification Assays
- Detection of HCV in Liver Tissue
- Detection of HCV RNA in Formalin-Fixed, Paraffin-Embedded Liver Tissue by RT-PCR
- Quantification of HCV RNA in Liver Tissue by bDNA Assay
- HCV Genotypes
- Hepatitis C Virus
- Molecular Evolutionary Analysis
- Determination of HCV-Specific Bulk CD8+ Activity in Liver
- Characterization of HCV-Specific Cytotoxic T-Lymphocytes from Liver Tissue
- Production of Human Monoclonal Antibodies to Hepatitis C Virus and Their Characterization
- In Vitro Culture Model
- Specific Detection of Negative Strand RNA of Hepatitis C Virus Using Chemical RNA Modification
- Strand-Specific rTth RT-PCR for the Analysis of HCV Replication
- Cell Culture Systems for the Detection of HCV Infection
- Replication and Detection of Hepatitis C Virus in Liver-Derived Cell Lines
- Primary Human Hepatocyte Culture for the Study of HCV
- A Cultivation Method for Highly Differentiated Primary Chimpanzee Hepatocytes Permissive for Hepatitis C Virus Replication
- Construction ofRecombinant Viral Vectors for the Expression of HCV Genes
- Construction of Recombinant Vaccinia Virus Expressing HCV Genes
- Construction of Recombinant Adeno-Associated Virus (AAV)
- Genotyping by Type-Specific Primers That Can Type HCV Types 1–6
- Genotyping Hepatitis C Virus by Type-Specific Primers for PCR Based on NS5 Region
- Determination of HCV Genotypes by RFLP
- HCV Genotyping by the Line Probe Assay INNO-LiPA HCV II
- Serological Genotyping Using Synthetic Peptides Derived from the NS4 Region
- Determination of HCV Quasispecies by Cloning and Sequencing
- Detection of Hepatitis C Virus Quasispecies Heterogeneity by Single-Strand Conformational Polymorphism
- Hepatitis C Virus Heteroduplex Tracking Assay
- Detection of HCV In Situ
- In Situ Detection of HCV
- In Situ Detection of Hepatitis C Viral Antigens
- In Situ Hybridization and the Detection and Localization of HCV RNA
- The In Situ Detection of PCR-Amplified Hepatitis C RNA
- lmmunoelectron Microscopic Characterization ofHCV
- Molecular Biologic Characterization
- Cloning and Assembly of Complex Libraries of Full-Length HCV cDNA Clones