Protein Acetylation : Methods and Protocols

The book focuses on the regulation of protein function and biological activity by the post-translational modification known as acetylation. Chapters detail a wide range of topics in protein acetylation dealing with pathways relevant to cellular homeostasis, gene expression, and DNA metabolism. Writt...

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Corporate Author: SpringerLink (Online service)
Other Authors: Brosh, Jr., Robert M. (Editor)
Format: eBook
Published: New York, NY Springer New York 2019, 2019
Edition:1st ed. 2019
Series:Methods in Molecular Biology
Online Access:
Collection: Springer eBooks 2005- - Collection details see MPG.ReNa
Table of Contents:
  • High-Resolution Mass Spectrometry to Identify and Quantify Acetylation Protein Targets
  • Isolation of Proteins on Nascent Chromatin and Characterization by Quantitative Mass Spectrometry
  • Detection and Quantitation of Acetylated Histones on Replicating DNA using In Situ Proximity Ligation Assay and Click-it Chemisty
  • Quantification of In Vitro Protein Lysine Acetylation by Reversed Phase HPLC
  • Measurement and Analysis of Lysine Acetylation by Kat Complexes In Vitro and In Vivo
  • Site-Specific Lysine Acetylation Stoichiometry across Subcellular Compartments
  • Lysine Acetylation of Proteins and its Characterization in Human Systems
  • Molecular and Cellular Characterization of SIRT1 Allosteric Activators
  • Assessment of SIRT2 Inhibitors in Mouse Models of Cancer
  • Using Yeast as a Model Organism to Study the Functional Roles of Histone Acetylation in DNA Excision Repair
  • Biochemical and Cellular Assays to Assess the Effects of Acetylation on Base Excision Repair Enzymes
  • Analysis of DNA Processing Enzyme FEN1 and its Regulation by Protein Lysine Acetylation
  • Examining the Role of HDACs in DNA Double-Strand Break Repair in Neurons
  • Experimental Approaches to Investigate the Role of Helicase Acetylation in Regulating R-Loop Stability
  • Rapid Detection of p53 Acetylation Status in Response to Cellular Stress Signaling
  • Isolation and Quantification Brain Region-Specific and Cell Subtype-Specific Histone (De)Acetylation in Cognitive Neuroepigenetics
  • Functional Analysis of HDACs in Tumorigeneis