Oligodendrocytes Methods and Protocols
This volume looks at the study of oligodendrocytes through in vitro and in vivo techniques, multiple model organisms, using approaches that bridge scales from molecular through system. Chapters in this book cover topics such as fundamental molecular analyses of oligodendrocytes and myelin; in vitro,...
| Other Authors: | , |
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| Format: | eBook |
| Language: | English |
| Published: |
New York, NY
Humana
2019, 2019
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| Edition: | 1st ed. 2019 |
| Series: | Methods in Molecular Biology
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| Subjects: | |
| Online Access: | |
| Collection: | Springer eBooks 2005- - Collection details see MPG.ReNa |
Table of Contents:
- Single-Cell RNA Sequencing of Oligodendrocyte Lineage Cells from the Mouse Central Nervous System
- Isolation and Preparation of Cells from Focal Remyelinating Central Nervous System Lesion sfor RNA Sequencing
- Myelin: Methods for Purification and Proteome Analysis
- In Vitro Generation and Electrophysiological Characterization of OPCc and Oligodendrocytes from Human Pluripotent Stem Cells
- Isolation and Culture of Oligodendrocytes
- A Neuron-Free Microfiber Assay to Assess Myelin Sheath Formation
- Oligodendrocyte-Neuron Myelinating Co-Culture
- Teasing of Ventral Spinal Cord White Matter Fibres for the Analysis of Central Nervous System Nodes of Ranvier
- Whole-Cell Patch Clamp Recordings from Oligodendrocyte Lineage Cells in Brain Slices
- Ex Vivo Slice Cultures to Study Myelination, Demyelination, and Remyelination in Mouse Brain and Spinal Cord
- Forward Genetic Screen using Zebrafish to Identify New Genes Involved in Myelination
- Manipulating Neuronal Activity in the Developing Zebrafish Spinal Cord to Investigate Adaptive Myelination
- A Drug Inducible Transgenic Zebrafish Model for Myelinating Glial Cell Ablation
- Conditional Demyelination and Remyelination in a Transgenic Xenopus laevis
- Conditional Mutagenesis in Oligodendrocyte Lineage Cells
- Recent Advances in Live Imaging of Cells of the Oligodendrocyte Lineage
- The DTA Mouse Model for Oligodentrocyte Ablation and CNS Demyelination
- Human Glial Chimeric Mice to Define the Role of Glial Pathology in Human Disease
- A Method to Detect Cytochrome c Oxidase Activity and Mitochondrial Proteins in Oligodencrocytes
- Transmission Electron Microscopy of Oligodendrocytes and Myelin
- Toxin-Based Models to Investigate Demyelination and Remyelination
- Magnetic Resonance techniques for Imaging White Matter