Hydrocarbon and Lipid Microbiology Protocols Meso- and Microcosms

This Volume describes methods for simulating natural environments by using reproducible and controllable meso- and microcosm experiments to analyse hydrocarbon-degrading microorganisms and to test hypotheses. It presents important aspects of the preparation of experimental set-ups, hydrocarbon appli...

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Bibliographic Details
Other Authors: McGenity, Terry J. (Editor), Timmis, Kenneth N. (Editor), Nogales, Balbina (Editor)
Format: eBook
Language:English
Published: Berlin, Heidelberg Springer Berlin Heidelberg 2017, 2017
Edition:1st ed. 2017
Series:Springer Protocols Handbooks
Subjects:
Online Access:
Collection: Springer eBooks 2005- - Collection details see MPG.ReNa
Table of Contents:
  • Introduction
  • Preparing the hydrocarbon / crude oil
  • High pressure cultivation of hydrocarbonoclastic aerobic bacteria
  • In vitro high pressure incubation and activity measurement of deep-sea, methanogenic archaea
  • Bioreactor design to emulate deep-sea hydrocarbon releases including formation of gas hydrates
  • Mudflat benthic spill simulations
  • Protocols for subtidal and deep sea benthic oil spill simulations
  • Simulation of anoxic/oxic oscillations in crude oil-degrading bioreactors
  • Protocols for microcosms for growing biofilms on hydrophobic substrates – a polyphasic approach to study biodiversity, metabolic activity and biofilm architecture
  • Application of micro- and mesocosms experiments to pollutant effects in biofilms
  • Mini sediment columns and two-dimensional sediment flow-through microcosms - versatile model systems for studying biodegradation of organic contaminants in groundwater ecosystems