Techniques to Investigate Mitochondrial Function in Neurons
This volume describes a broad spectrum of experimental approaches for investigating structure, function, and transport of neuronal mitochondria in health and disease. Most of these approaches were only recently developed and range from electron tomography-based 3D reconstruction of mitochondrial cri...
| Other Authors: | , |
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| Format: | eBook |
| Language: | English |
| Published: |
New York, NY
Humana
2017, 2017
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| Edition: | 1st ed. 2017 |
| Series: | Neuromethods
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| Subjects: | |
| Online Access: | |
| Collection: | Springer eBooks 2005- - Collection details see MPG.ReNa |
Table of Contents:
- Three-Dimensional Reconstruction of Neuronal Mitochondria by Electron Tomography
- Measuring Mitochondrial Shape with ImageJ
- Live Imaging Mitochondrial Transport in Neurons
- Techniques to Investigate Bioenergetics of Mitochondria
- Respirometry in Neurons
- Measuring ATP in Axons with FRET
- Characterizing Metabolic States using Fluorescence Lifetime Imaging Microscopy (FLIM) of NAD(P)H
- Techniques for Simultaneous Mitochondrial and Cytosolic Ca2+ Imaging in Neurons
- Live Imaging of Mitochondrial ROS Production and Dynamic Redox Balance in Neurons
- Monitoring of Permeability Transition Pore Openings in Isolated Individual Brain Mitochondria
- Examination of Mitochondrial Ion Conductance by Patch Clamp in Intact Neurons and Mitochondrial Membrane Preparations
- Monitoring of Permeability Transition Pore Openings in Mitochondria of Cultured Neurons
- Protocols for Assessing Mitophagy in Neuronal Cell Lines and Primary Neurons
- Examining Mitochondrial Function at Synapses In Situ
- Proteomic Analysis of Neuronal Mitochondria
- Measuring Mitochondrial Pyruvate Oxidation