Human hepatocyte isolation for clinical transplantation
| Main Author: | |
|---|---|
| Format: | eBook |
| Language: | English |
| Published: |
London
Henry Stewart Talks
2014, 2014
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| Series: | Stem cells : recent advances in understanding and utilizing
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| Subjects: | |
| Online Access: | |
| Collection: | Henry Stewart Talks - Collection details see MPG.ReNa |
Table of Contents:
- GMP level cell isolation from pediatric liver
- Liver perfusion
- Tissue dissociation
- Cell suspension
- Filtering into collection bags and waste transpacks
- Final product of hepatocyte pellet
- Testing final cell product for bacteria and fungus
- Dispersing cells into aliquots for infusion
- Cold storage and post cold suspension of hepatocytes
- Hepatocyte transplant procedure & Cell infusion