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Drosophila Oogenesis Methods and Protocols

This volume provides current up-to-date protocols for preparing the ovary for various imaging techniques, genetic protocols for generating mutant clones, mosaic analysis and assessing cell death. Chapters address methods for performing genome wide gene expression analysis and bioinformatics for stud...

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Bibliographic Details
Other Authors: Bratu, Diana P. (Editor), McNeil, Gerard P. (Editor)
Format: eBook
Language:English
Published: New York, NY Humana 2015, 2015
Edition:1st ed. 2015
Series:Methods in Molecular Biology
Subjects:
Cell Biology
Cytology
Experimental Organisms
Biology / Technique
Online Access:
Collection: Springer eBooks 2005- - Collection details see MPG.ReNa
Table of Contents:
  • Drosophila melanogaster Oogenesis: An Overview
  • Basic Techniques in Drosophila Ovary Preparation
  • Mosaic Analysis in the Drosophila Melanogaster Ovary
  • Genetic Mosaic Analysis of Stem Cell Lineages in the Drosophila Ovary
  • Culturing Drosophila Egg Chambers and Investigating Developmental Processes through Live Imaging
  • Border Cell Migration: A Model System for Live Imaging and Genetic Analysis of Collective Cell Movement
  • Visualizing Microtubule Networks during Drosophila Oogenesis using Fixed and Live Imaging­­Visualization of Actin Cytoskeletal Dynamics in Fixed and Live Drosophila Egg Chambers
  • Single Molecule RNA in situ Hybridization (smFISH) and Immunofluorescence (IF) in the Drosophila Egg Chamber
  • Fluorescent In Situ Hybridization of Nuclear Bodies in Drosophila melanogaster Ovaries
  • Ultrastructural Analysis of Drosophila Ovaries by Electron Microscopy
  • Immuno-Electron Microscopy and Electron Microscopic in situ Hybridization for Visualizing piRNA Biogenesis Bodies in Drosophila Ovaries
  • Visualizing Cytoophidia Expression in Drosophila Follicle Cells via Immunohistochemistry.-Detection of Cell Death and Phagocytosis in the Drosophila Ovary
  • Analysis of Cell Cycle Switches in Drosophila Oogenesis
  • Global Run-On Sequencing (GRO-seq) Library Preparation from Drosophila Ovaries
  • Bioinformatics Analysis to Identify RNA-protein Interactions in Oogenesis

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