Plant Functional Genomics Methods and Protocols
This second edition volume discusses the revolutionary development of faster and less expensive DNA sequencing technologies from the past 10 years and focuses on general technologies that can be utilized by a wide array of plant biologists to address specific questions in their favorite model system...
| Other Authors: | , |
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| Format: | eBook |
| Language: | English |
| Published: |
New York, NY
Humana
2015, 2015
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| Edition: | 2nd ed. 2015 |
| Series: | Methods in Molecular Biology
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| Subjects: | |
| Online Access: | |
| Collection: | Springer eBooks 2005- - Collection details see MPG.ReNa |
Table of Contents:
- Analysis and Visualization of RNA-Seq Expression Data Using RStudio, Bioconductor, and Integrated Genome Browser.-Constructing Simple Biological Networks for Understanding Complex High-Throughput Data in Plants
- Targeted Plant Genome Editing via the CRISPR/Cas9 Technology
- QTL Mapping Using High-Throughput Sequencing
- Quantitating Plant MicroRNA-Mediated Target Repression Using a Dual-Luciferase Transient Expression System
- Persistent Virus-Induced Gene Silencing in Asymptomatic Accessions of Arabidopsis
- A User’s Guide to the Arabidopsis T-DNA Insertion Mutant Collections
- Genome-Wide Association Mapping in Plants Exemplified for Root Growth in Arabidopsis thaliana
- Tilling By Sequencing
- SHOREmap v3.0: Fast and Accurate Identification of Causal Mutations from Forward Genetic Screens
- Software-Assisted Stacking of Gene Modules Using GoldenBraid 2.0 DNA-Assembly Framework
- Ligation Independent Cloning for Plant Research
- Gene Functional Analysis Using Protoplast Transient Assays
- Descriptive vs. Mechanistic Network Models in Plant Development in the Post-Genomic Era
- Epigenome Profiling of Specific Plant Cell Types Using a Streamlined INTACT Protocol and ChIP-seq
- Whole-Genome DNA Methylation Profiling with Nucleotide Resolution
- High-Throughput Nuclease-Mediated Probing of RNA Secondary Structure in Plant Transcriptomes
- Genome-Wide Mapping of DNase I Hypersensitive Sites in Plants
- Characterization of in-vivo DNA-Binding Events of Plant Transcription Factors by ChIP-seq: Experimental Protocol and Computational Analysis
- Identification of Direct Targets of Plant Transcription Factors Using the GR Fusion Technique
- Ribosome Profiling: A Tool for Quantitative Evaluation of Dynamics in mRNA Translation
- Tissue-Specific Gene Expression Profiling by Cell Sorting
- Translating Ribosome Affinity Purification (TRAP) Followed by RNA Sequencing Technology (TRAP-SEQ) for Quantitative Assessment of Plant Translatomes
- Rapid Immunopurification of Ribonucleoprotein Complexes of Plants
- Metabolomic Profiling of Plant Tissues