Stem Cells and Good Manufacturing Practices Methods, Protocols, and Regulations

This volume collects a series of protocols describing the kinds of infrastructures, training, and standard operating procedures currently available to actualize the potential of stem cells for regenerative therapies. Stem Cells and Good Manufacturing Practices: Methods, Protocols, and Regulations pu...

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Bibliographic Details
Other Authors: Turksen, Kursad (Editor)
Format: eBook
Language:English
Published: New York, NY Humana 2015, 2015
Edition:1st ed. 2015
Series:Methods in Molecular Biology
Subjects:
Online Access:
Collection: Springer eBooks 2005- - Collection details see MPG.ReNa
Table of Contents:
  • Therapeutic Mesenchymal Stromal Cells: Where We Are Headed
  • The Suspension Culture of Undifferentiated Human Pluripotent Stem Cells Using Spinner Flasks
  • Scalable Expansion of Human Induced Pluripotent Stem Cells in Xeno-Free Microcarriers
  • Derivation of GMP-Compliant Integration-Free hiPSCs Using Modified mRNAs
  • Labeling Pluripotent Stem Cell-Derived Neural Progenitors with Iron Oxide Particles for Magnetic Resonance Imaging
  • Using the Quantum Cell Expansion System for the Automated Expansion of Clinical-Grade Bone Marrow-Derived Human Mesenchymal Stromal Cells
  • Isolation and Expansion of Mesenchymal Stromal/Stem Cells from Umbilical Cord Under Chemically Defined Conditions
  • Production of Good Manufacturing Practice-Grade Human Umbilical Cord Blood-Derived Mesenchymal Stem Cells for Therapeutic Use
  • Xeno-Free Culture of Human Periodontal Ligament Stem Cells
  • GMP-Compliant Human Adipose Tissue-Derived Stromal Cells for Cellular Therapy
  • cGMP-Compliant Transportation Conditions for a Prompt Therapeutic Use of Marrow Mesenchymal Stromal/Stem Cells
  • GMP-Grade Human Fetal Liver-Derived Mesenchymal Stem Cells for Clinical Transplantation
  • Purification of Human Induced Pluripotent Stem Cell-Derived Neural Precursors Using Magnetic Activated Cell Sorting
  • Scalable Ex Vivo Expansion of Human Mesenchymal Stem/Stromal Cells in Microcarrier-Based Stirred Culture Systems
  • Media Fill for Validation of a Good Manufacturing Practice-Compliant Cell Production Process
  • Standard Operating Procedure for the Good Manufacturing Practice-Compliant Production of Human Bone Marrow Mesenchymal Stem Cells
  • Culture of Human Limbal Epithelial Stem Cells on Tenon’s Fibroblast Feeder-Layers: A Translational Approach
  • Bioreactor Expansion of Human MesenchymalStem Cells According to GMP Requirements