Structural Proteomics High-Throughput Methods

This updated and expanded volume reflects the current state of the structural protein field with improved and refined protocols that have been applied to particularly challenging proteins, notably integral membrane proteins and multi-protein complexes. Structural Proteomics: High-Throughput Methods,...

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Bibliographic Details
Other Authors: Owens, Raymond J. (Editor)
Format: eBook
Language:English
Published: New York, NY Humana 2015, 2015
Edition:2nd ed. 2015
Series:Methods in Molecular Biology
Subjects:
Online Access:
Collection: Springer eBooks 2005- - Collection details see MPG.ReNa
Table of Contents:
  • Protein Structure Annotation Resources
  • PiMS: A Data Management System for Structural Proteomics
  • Prediction and Analysis of Intrinsically Disordered Proteins
  • I-TASSER Suite: A Standalone Package for Protein Structure Prediction and Structure-Based Protein Function Annotation
  • Characterization and Production of Protein Complexes by Co-Expression in Escherichia coli
  • The Production of Multi-Protein Complexes in Insect Cells Using the Baculovirus Expression System
  • Production of Cell Surface and Secreted Glycoproteins in Mammalian Cells
  • Cell-Free Protein Synthesis Systems Derived from Cultured Mammalian Cells
  • Crystallization: Digging into the Past to Learn Lessons for the Future
  • Screening of Stable G Protein-Coupled Receptor Variants in Saccharomyces cerevisiae
  • Cell-Free Expression of G Protein-Coupled Receptors
  • GFP-Based Expression Screening of Membrane Proteins in Insect Cells Using the Baculovirus System
  • Methods for the Successful Crystallization of Membrane Proteins
  • Application of In Situ Diffraction in High-Throughput Structure Determination Platforms
  • CD Spectroscopy: An Essential Tool for Quality Control of Protein Folding
  • High Throughput Studies of Protein Shapes and Interactions by Synchrotron Small-Angle X-Ray Scattering
  • Automated Structure Determination from NMR Spectra
  • Solid State Nuclear Magnetic Resonance Spectroscopy for Membrane Protein Structure Determination
  • Native Mass Spectrometry: Towards High-Throughput Structural Proteomics