Membrane Proteins Production for Structural Analysis
This volume reviews the latest development in production, stabilization and structural analysis techniques of membrane proteins. It contains 14 chapters exploring topics including the advances in heterologous expression systems, stabilization tools and structural methods that contributed to the grow...
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| Format: | eBook |
| Language: | English |
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New York, NY
Springer New York
2014, 2014
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| Edition: | 1st ed. 2014 |
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| Online Access: | |
| Collection: | Springer eBooks 2005- - Collection details see MPG.ReNa |
Table of Contents:
- 1 Membrane Proteins Production for Structural Analysis
- 2 Membrane Protein Quality Control in Cell-Free Expression Systems: Tools, Strategies and Case Studies
- 3 Bacterial Expression and Stabilization of GPCRs
- 4 Membrane Protein Production in Escherichia Coli: Overview and Protocols
- 5 Lactococcus Lactis, Recent Developments in Functional Expression of Membrane Proteins
- 6 Over expression of Membrane Proteins in Saccharomyces Cerevisiae for Structural and Functional Studies: a Focus on the Rabbit Ca2+-ATPase Serca1a and on the Yeast Lipid “Flippase” Complex Drs2p/Cdc50p
- 7 Amphipols: a General Introduction and Some Protocols
- 8 New Amphiphiles to Handle Membrane Proteins: “Ménage à Trois” between Chemistry, Physical-Chemistry and Biochemistry
- 9 Building Model Membranes with Lipids and Proteins: Dangers and Challenges
- 10 Analytical Ultracentrifugation and Size Exclusion Chromatography Coupled to Light Scattering for the Characterization of Membrane Proteins in Solution
- 11 Lipidic Cubic Phase Technologies for Structural Studies of Membrane Proteins
- 12 Micelles, Bicelles, Amphipols, Nano discs, Liposomes or Intact Cells: the Hitchhiker's Guide to the Membrane Protein Study by NMR
- 13 Foundations of Bio molecular Simulations: A Critical Introduction to Homology Modeling, Molecular Dynamics Simulations and Free Energy Calculations of Membrane Proteins
- 14 Structural Studies of TSPO, a Mitochondrial Membrane Protein