Human Cytomegaloviruses Methods and Protocols

With the significant medical role that human cytomegalovirus plays in human disease (from acute disease following primary infection, to chronic disease due to life-long viral persistence), the need to better understand human cytomegalovirus biology and pathogenesis is needed.  Human Cytomegaloviruse...

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Bibliographic Details
Other Authors: Yurochko, Andrew D. (Editor), Miller, William E. (Editor)
Format: eBook
Language:English
Published: Totowa, NJ Humana 2014, 2014
Edition:1st ed. 2014
Series:Methods in Molecular Biology
Subjects:
Online Access:
Collection: Springer eBooks 2005- - Collection details see MPG.ReNa
Table of Contents:
  • History of the Molecular Biology of Cytomegalovirus
  • Overview of Human Cytomegalovirus Pathogenesis
  • Distinct Properties of Human Cytomegalovirus Strains and the Appropriate Choice of Strains for Particular Studies
  • Use of Diploid Human Fibroblasts as a Model System to Culture/Grow, Study and Understand Human Cytomegalovirus Infection
  • Use of Recombinant Approaches to Construct Human Cytomegalovirus Mutants
  • The Use of Primary Human Cells (Fibroblasts, Monocytes and Others) to Assess Human Cytomegalovirus Function
  • Human Hematopoietic Long-Term Culture (hLTC) for Human Cytomegalovirus Latency and Reactivation
  • Analysis of Cytomegalovirus Binding/Entry-Mediated Events
  • Use of 5-Ethynyl-2’-Deoxyuridine Labelling and Flow Cytometry to Study Cell Cycle-Dependent Regulation of Human Cytomegalovirus Gene Expression
  • Methods for Studying the Function of Cytomegalovirus GPCRs
  • Methods for the Detection of Cytomegalovirus in Glioblastoma Cells and Tissues
  • Methods to Study the Nucleocytoplasmic Transport of Macromolecules with Respect to Their Impact on the Regulation of Human Cytomegalovirus Gene Expression
  • Fluorescence-Based Laser Capture Microscopy Technology Facilitates Identification of Critical In Vivo Cytomegalovirus Transcriptional Programs
  • Techniques for Characterizing Cytomegalovirus-Encoded miRNAs
  • What We Have Learned From Animal Models of HCMV
  • Rodent Models of Congenital Cytomegalovirus Infection
  • Recent Approaches and Strategies in the Generation of Anti-HumanCytomegalovirus Vaccines
  • Approaches for the Generation of New Anti-Cytomegalovirus Agents:  Identification of Protein-Protein Interaction Inhibitors and Compounds Against the HCMV IE2 Protein