Electrotransformation of Bacteria

Creating bacteria with modified genetic properties allows the specific investigation of these microorganisms. Electrotransformation is a highly efficient and easy to apply technique to introduced genetic material into bacterial cells, with the use of a strong electric field. In the present manual, p...

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Corporate Author: SpringerLink (Online service)
Other Authors: Eynard, Natalie (Editor), Teissie, Justin (Editor)
Format: eBook
Language:English
Published: Berlin, Heidelberg Springer Berlin Heidelberg 2000, 2000
Edition:1st ed. 2000
Series:Springer Lab Manuals
Subjects:
Online Access:
Collection: Springer Book Archives -2004 - Collection details see MPG.ReNa
Summary:Creating bacteria with modified genetic properties allows the specific investigation of these microorganisms. Electrotransformation is a highly efficient and easy to apply technique to introduced genetic material into bacterial cells, with the use of a strong electric field. In the present manual, protocols for the transformation of about 40 strains of bacteria are described. Emphasis is given on the individual critical procedural steps, since the practical details depend on the bacterial strain under investigation. This presentation together with the theoretical introductionary chapters, allows the user to modify and adapt each protocol to his/her own experiments. Bacterial strains with relevance in the food industry and biotechnology, in medical and veterinary fields, agroindustry and environmental sciences are covered
Physical Description:XI, 292 p. 8 illus online resource
ISBN:9783662043059