Optical Activity of Proteins and Other Macromolecules
The application of circular dichroism (CD) to various problems involving con formation of proteins and other biopolymers is emphasized in this revised and enlarged second edition. The usefulness of CD and ORD in helping to solve structural prob lems is demonstrated by many examples, and the most e...
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| Format: | eBook |
| Language: | English |
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Berlin, Heidelberg
Springer Berlin Heidelberg
1973, 1973
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| Edition: | 2nd ed. 1973 |
| Series: | Molecular Biology, Biochemistry and Biophysics Molekularbiologie, Biochemie und Biophysik
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| Subjects: | |
| Online Access: | |
| Collection: | Springer Book Archives -2004 - Collection details see MPG.ReNa |
Table of Contents:
- I The Realm of Proteins. Structural Features. The Phenomenon of Optical Activity. Historical Highlights
- II The Phenomena of Optical Activity. Terms and Definitions. Theoretical Considerations. The Drude and Moffitt Equations
- 1. Refraction of Monochromatic Polarized Light
- 2. Optical Rotation
- 3. Specific Rotation
- 4. Corrected Mean Residual Specific Rotation
- 5. Optical Activity and Absorption of Light
- 6. Optical Rotatory Dispersion. The Drude Equation
- 7. The Moffitt Equation for the Rotatory Dispersion of Helical Structures
- 8. Configuration and Conformation
- 9. The Far Ultraviolet Absorption Maxima and Cotton Effects
- 10. Absorption and Refraction
- 11. Circular Dichroism
- 12. Magnetic Circular Dichroism
- III Polarimeters and Spectropolarimeters. The Measurement of Optical Activity
- 1. Visual Polarimetry
- 2. Photoelectric Spectropolarimetry
- 4. Amino Acid Composition and Conformation of Proteins
- VI The Far Ultraviolet Cotton Effects of Synthetic Polyamino Acids
- 1. The ?-Helical and Random Conformations of Poly-?-l-glutamic Acid and Poly-?-l-lysine
- 2. ORD of Other Structural Orders
- 3. Circular Dichroism of Polyamino Acids of Various Conformations
- VII The Cotton Effects and Conformation of Proteins
- 1. Attempts to Classify Proteins According to Their Conformation
- 2. Comments on Nomenclature
- 3. Cotton Effects of Proteins with a High ?-Helix Content
- 4. Rotatory Dispersion, Circular Dichroism, X-Ray Diffraction, and Conformation of Myoglobin, Lysozyme, and Other Proteins
- 5. Conformational Transitions and Cotton Effects in the Near Ultraviolet and Visible Spectrum
- 6. The Fine Structure of the Near Ultraviolet CD Bands and Conformational Features of the Aromatic Chromophores
- VIII Cotton Effects and Conformation of Nonhelical Proteins
- 1. Globular Proteins of Known and Unknown Conformation
- 3. Optical Rotatory Dispersion Measurement. General Principles of Operation. Sources of Errors. Accuracy and Reproducibility
- 4. Cells and Solutions
- 5. Circularly Polarized Light
- 6. Circular Dichroism Measurement
- 7. Resolution of Circular Dichroism Curves
- 8. Circular Dichroism Measurement in the Vacuum Ultraviolet Region
- IV Optical Activity of Amino Acids, Peptides, and Proteins
- 1. Optical Rotation and Configuration of ?-Amino Acids
- 2. Optical Rotatory Dispersion of ?-Amino Acids
- 3. Rotatory Contribution of Amino Acid Residues in Peptides
- 4. Optical Rotation of Proteins at Various pH
- 5. Circular Dichroism of Amino Acids
- V The Optical Rotatory Dispersion of Polyamino Acids and Proteins. Measurements in the Visible and Near Ultraviolet Spectral Zones
- 1. Rotatory Dispersion of Polyamino Acids as Protein Models
- 2. Rotatory Dispersion of Proteins
- 3. Limitations of the Drude and MoffittMethods in the Elucidation of Protein Conformation
- XI Optical Activity of Glycoproteins and Lipoproteins
- 1. Glycoproteins and Polysaccharides
- 2. Lipoproteins
- 3. Membranes and Other Particulate Suspensions
- XII Concluding Remarks
- References
- 2. Globular Proteins having a High Content of ? Conformation
- 3. Cotton Effects and Conformation of Bovine Pancreatic Ribonuclease
- 4. Other Rigid Nonhelical Proteins
- 5. Cotton Effects and Conformation of Flexible Nonhelical Proteins
- 6. Cotton Effects and Conformation of Immunoglobulins
- 7. Effect of Detergents on the Conformation of Nonhelical Proteins
- 8. Circular Dichroism of Small Polypeptide Hormones
- IX Optical Activity of Structural Proteins
- 1. Natural Variety of Structural Proteins and Difficulties Involved in Their Study
- 2. Structural Proteins with a High ?-Helix Content
- 3. Optical Activity of Structural Proteins with ? Conformation
- 4. Optical Activity and Conformation of Collagen
- X Optical Activity of Nucleoproteinsand Histones
- 1. Conformation of Histones
- 2. Optical Activity of Nucleic Acids and Nucleoproteins
- 3. Conformation of Proteins and Nucleic Acids in Ribosomes, Viruses, and Phages