Animal Cell Culture Techniques
Cell culture techniques allow a variety of molecular and cell biological questions to be addressed, offering physiological conditions whilst avoiding the use of laboratory animals. In addition to basic techniques, a wide range of specialised practical protocols covering the following areas are inclu...
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| Format: | eBook |
| Language: | English |
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Berlin, Heidelberg
Springer Berlin Heidelberg
1998, 1998
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| Edition: | 1st ed. 1998 |
| Series: | Springer Lab Manuals
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| Subjects: | |
| Online Access: | |
| Collection: | Springer Book Archives -2004 - Collection details see MPG.ReNa |
Table of Contents:
- I General Methods
- 1 Cloning Animal Cells
- 2 Cell Culture and Diagnostic Virology
- 3 Screening for Mycoplasma Contamination in Animal Cell Cultures
- 4 Serum-Free Media
- 5 Screening Culture Media and Sera for Endotoxin Testing
- 6 Generation of Monoclonal Antibodies and Characterization of Novel Antibodies by Western Blotting and Immunocytochemistry
- 7 The Generation of Long-Term Human Antibody-Producing Cell Lines Using the Epstein-Barr Virus
- 8 Primary Culture
- II Cell proliferation and Death
- 9 Baculoviral Expression and Partial Purification of Cyclin/CDK Complexes
- 10 Methods for Studying Phosphoinositide Metabolism in Cultured Cells
- 11 Analysis of Cell Cycle and Cell Death Mechanisms
- III In Vitro Models for Cell Differentiation
- 12 Assessing Release of Secretory Products from Individual Cells
- 13 The Granulocyte/Macrophage Colony-Forming Unit Assay
- 14 Investigations into Cell Differentiation Using Cells in Culture
- 15 Cell Culture Models for Mononuclear Phagocytes
- 16 The Human Leukemia Cell Line HL-60 as a Cell Culture Model To Study Neutrophil Functions and Inflammatory Cell Responses
- 17 Cell Culture Models for Polarized Epithelial Monolayers
- 18 Ion Channels and Cell Signaling in Cell Cultures
- 19 Isolation, Cultivation and Differentiation of Lung Type II Epithelial Cells
- 20 Culture Systems for Hepatocytes for Use in Toxicology and Differentiation Studies
- IV In Vitro Models for Toxicology and Pharmacology
- 21 Cellular Models for In Vitro Toxicity Testing
- 22 Miniaturized In Vitro Methods in Toxicity Testing
- 23 Investigation of Anti-tumor Drug Accumulation and Efflux in Cell Culture
- 24 Current Status of Genetically Engineered In Vitro Systems for Biotransformation
- V Industrial Application of Animal Cell Culture
- 25 Scale-Up of Animal Cell Cultures
- 26 Purification of Monoclonal Antibodies Produced by Animal Cells in Culture
- 27 Biosafety Assessment of Cultured Animal Cells Used for Biopharmaceutical Production
- 28 Cytokine Cell Culture Assays
- VI Genetic Manipulation and Analysis of Human and Animal Cells in Culture
- 29 Antisense Methods in Cell Culture
- 30 Transfection with Ribozymes to Investigate the Role of Specific Gene Expression
- 31 Methods for Studying the Transcription and DNase I Hypersensitive Sites of Genes in Cells in Culture
- 32 Analysis of Specific and Differentially Expressed mRNAs in Cell Culture
- 33 Human Gene Therapy: Review and Outlook