High Performance Liquid Chromatography
Modern liquid column chromatography (LC) has developed rapidly since 1969 to become a standard method of separation. If the statisticians are to be believed, the recent growth of LC has been the most specta cular development in analytical chemistry and has not yet abated be cause its vast potentia...
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| Format: | eBook |
| Language: | English |
| Published: |
Berlin, Heidelberg
Springer Berlin Heidelberg
1979, 1979
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| Edition: | 1st ed. 1979 |
| Series: | Chemical Laboratory Practice
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| Subjects: | |
| Online Access: | |
| Collection: | Springer Book Archives -2004 - Collection details see MPG.ReNa |
Table of Contents:
- I. Chromatographic Processes
- References Chapter I
- II. Fundamentals of Chromatography
- A. Retention
- B. Linear Flow Rate, Porosity, Permeability
- C. Band Broadening
- D. Resolution
- E. Dependence of Band Broadening on Flow Rate
- F. Band Broadening and Particle Size
- G. Extra-Column Band Broadening
- H. Optimum Analysis Conditions and Analysis Time
- I. Selection of a Suitable Column
- References Chapter II
- III. Equipment for HPLC
- A. Solvent Reservoir — Degassing of the Eluent
- B. Pumps
- C. Damping of the Pulsations
- D. Sample Introduction
- E. The Column
- F. Thermostating
- G. Measurement of the Flow Rate
- H. Fraction Collectors
- I. Recorders
- J. Instrumentation for Gradient Elution
- K. Safety Measures
- References Chapter III
- IV. Detectors
- A. UV Detectors
- B. Differential Refractometer
- C. Microadsorption Detector
- D. Transport Detector (Flame Ionization Detector)
- E. Fluorescence Detector
- F. Other Detectors
- A. Introduction
- B. Basics of Exclusion Chromatography
- C. Stationary Phases for Exclusion Chromatography
- D. Applications of Exclusion Chromatography
- References Chapter IX
- X. Selection of the Separation System
- References Chapter X
- XI. Special Techniques
- A. Preparative Chromatography
- B. Qualitative Analysis
- C. Quantitative Analysis
- D. Trace Analysis
- References Chapter XI
- XII. Purification of Solvents
- References Chapter XII.
- G. Comparison of the Important Detectors
- H. Reaction Detectors
- References Chapter IV
- V. Stationary Phases
- A. Packing Materials for Adsorption and Partition Chromatography
- B. Chemically Modified Supports
- C. Ion Exchangers
- D. Stationary Phases for Exclusion Chromatography
- References Chapter V
- VI. Adsorption Chromatography
- I. Polar Stationary Phases
- II. Nonpolar Stationary Phases
- III. The General Elution Problem
- IV. Applications of Adsorption Chromatography
- References Chapter VI
- VII. Partition Chromatography
- A. Introduction
- B. Supports and Liquid Phases
- C. Properties of the Column
- D. Applications
- E. Ion-pair Chromatography
- References Chapter VII
- VIII. Ion-Exchange Chromatography
- A. Principle
- B. Ion Exchange Materials
- C. Characterization of the Ion Exchangers
- D. Optimizing aSeparation
- E. Applications
- References Chapter VIII
- IX. Exclusion Chromatography. Gel Permeation Chromatography