Advanced Techniques in Biological Electron Microscopy
The past decade has seen a remarkable increase in the use of electron microscopy as a researm tool in biology and medicine. Thus, most institu tions of higher learning now boast several electron optical laboratories having various levels of sophistication. Training in the routine use of elec tron...
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| Format: | eBook |
| Language: | English |
| Published: |
Berlin, Heidelberg
Springer Berlin Heidelberg
1973, 1973
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| Edition: | 1st ed. 1973 |
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| Online Access: | |
| Collection: | Springer Book Archives -2004 - Collection details see MPG.ReNa |
Table of Contents:
- Embedding Media — Old and New
- A. Introduction
- B. Early Embedding Media
- C. Conventional Embedding Media
- D. Advantages and Disadvantages of Conventional Embedding Media
- E. New Embedding Media
- F. Conclusion
- References
- Substitution Techniques
- A. Introduction
- B. Inert-dehydration
- C. Freeze-substitution
- D. Conclusions
- References
- Freeze-Etching and Freeze-Fracturing
- A. Introduction
- B. Freezing of Biological Systems
- C. Methods and Instrumentation
- D. Interpretation
- E. Conclusions
- References
- Electron Microscope Autoradiography
- F. A. McHenry
- A. Introduction
- B. Distribution of Developed Grains Around Radioactive Sources
- C. Analysis of Autoradiograms
- D. Conversion of Developed Grain Data to Information on Radioactivity
- References
- Scanning Electron Microscope Techniques in Biology
- A. Introduction
- B. Specimen Preparation
- C. Viewing Techniques
- D. Signal Processing
- E. Recording Techniques
- F. Information Assimilation by the Observer
- 3. Electrical Properties and Charging
- G. Conclusion
- H. Appendices
- I. Optical Aids for the Viewing of Vertically Mounted Stereo-Pairs
- References
- Computer Processing of Electron Micrographs
- A. Introduction
- B. Linear Systems and Fourier Processing
- C. Digitizing of Electron Micrographs
- D. Noise Filtering
- E. The Cross Correlation Function and its Use for Image Alignment
- F. Two-Dimensional Restoration
- G. Object/Support Separation
- H. Three-Dimensional Reconstruction
- References
- High Voltage Electron Microscppy
- A. Introduction
- B. Merits of the High Voltage Electron Microscope
- C. Biological Applications
- D. Design and Construction of High Voltage Electron Microscopes
- References