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Plant Cell Wall Analysis

Modern Methods of Plant Analysis When the handbook Modern Methods of Plant Analysis, was first introduced in 1954, the considerations were: 1. the dependence of scientific progress in biology on the improvement of existing and the introduction of new methods; 2. the difficulty in finding many new an...

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Bibliographic Details
Other Authors: Linskens, Hans F. (Editor), Jackson, John F. (Editor)
Format: eBook
Language:English
Published: Berlin, Heidelberg Springer Berlin Heidelberg 1996, 1996
Edition:1st ed. 1996
Series:Molecular Methods of Plant Analysis
Subjects:
Cell Biology
Cytology
Botany
Biotechnology
Biochemistry
Plant Science
Online Access:
Collection: Springer Book Archives -2004 - Collection details see MPG.ReNa
Table of Contents:
  • Analysis of Chitin Biosynthesis
  • 1 Introduction
  • 2 Structure of Chitin
  • 3 The Enzymatic Synthesis of Chitin
  • 4 Preparation of Chitin Synthase
  • 5 Product Characterization of Chitin Synthase
  • 6 Polymerization and Crystallization
  • 7 Fungal Chitin Synthase Genes
  • 8 Morphogenetic Roles of Chitin Synthases
  • 9 Conclusions
  • References
  • Analysis of Plant-Substratum Adhesives
  • 1 Introduction
  • 2 Case Studies of Cell-Substratum Adhesion
  • 3 Methods for the Analysis of Cell-Substratum Adhesives
  • 4 Conclusions
  • References
  • Biochemical, Immunological and Molecular Analyses of Extensin
  • 1 Introduction
  • 2 Biochemical Characterization of Extensin
  • 3 Immunological Detection of Extensin
  • 4 Molecular Characterization of Extensin
  • 5 Summary
  • References
  • Analysis of Pectin Structure by HPAEC-PAD
  • 1 Introduction
  • 2 Pectin HPLC Separations
  • 3Pectin Analysis in Ripening Peach Fruit
  • 4 Conclusions and Future Directions
  • References
  • Fractionation of Cell Wall Components
  • 1 Introduction
  • 2 Polysaccharides
  • 3 Proteins and Glycoproteins
  • 4 Lignins
  • References
  • Isolation and Analysis of Cell Wall Polymers from Olive Pulp
  • 1 Introduction
  • 2 Isolation of Cell Walls from Olive Pulp
  • 3 Sequential Extraction of Cell Wall Polymers
  • 4 Fractionation of the Extracted Cell Wall Polysaccharides
  • 5 Chemical and Spectroscopic Analysis
  • 6 Concluding Remarks
  • References
  • Determination of Cell Wall Autolysis
  • 1 Introduction
  • 2 Factors Altering Cell Wall Isolation and Autolysis
  • 3 Isolation of Active Cell Walls
  • 4 Isolation of Inactive Cell Walls (Controls)
  • 5 Autolysis Incubations
  • 6 Analysis of Autolysis Products
  • 7 Conclusions
  • References
  • Cell Wall Porosity and Its Determination
  • 1 Introduction
  • 2 Microscopic Visualization of Wall Pores
  • 3 Bulk Exclusion Techniques
  • 4 Tracer Techniques: Uptake of Molecules or Particles
  • 5 Conclusions
  • References
  • 5 Summary
  • References
  • Characterization of Oligosaccharides Derived from Plant Cell Wall Polysaccharides by On-Line High-Performance Anion-Exchange Chromatography Thermospray Mass Spectrometry
  • 1 Introduction
  • 2 Methods
  • 3 HPAEC in Oligosaccharide Analysis
  • 4 General Experimental Considerations Related to HPAEC-MS
  • 5 Application of HPAEC-MS in Oligosaccharide Characterization
  • 6 Conclusions and Perspectives
  • References
  • Analysis of Pectin Methyl Esterases
  • 1 Introduction
  • 2 Estimation and Detection of PME Activities
  • 3 PME Localization
  • 4 PME Extraction and Purification
  • 5 PME Properties
  • 6 Roles of PMEs
  • 7 Conclusions and Perspectives
  • References
  • Probing the Subunit Composition and Topology of Plasma Membrane-Bound (1,3)-?-Glucan (Callose) Synthases
  • 1 Introduction
  • 2 Membrane Isolation, Enzyme Assay, and Solubilization
  • 3 Callose Synthase Topology, Purification, and Subunit Composition
  • 4 Biochemical Characterization of Integral Plant Plasma Membrane Proteins

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